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Literature summary for 3.1.3.74 extracted from

  • Jang, Y.M.; Kim, D.W.; Kang, T.C.; Won, M.H.; Baek, N.I.; Moon, B.J.; Choi, S.Y.; Kwon, O.S.
    Human pyridoxal phosphatase. Molecular cloning, functional expression, and tissue distribution (2003), J. Biol. Chem., 278, 50040-50046.
    View publication on PubMed
Search for more literature for 3.1.3.74

Cloned(Commentary)

Cloned (Comment) Organism
cDNA, from brain, expression in Escherichia coli M15/pRER4, sequencing, ORF is located on chromosome 22q12.3, genomic organization Homo sapiens
full-length cDNA, from brain, sequencing, ORF is located on chromosome 15.E1, genomic organization Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 pyridoxal 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.0434
-
 pyridoxine 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.0806
-
 pyridoxamine 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement for a divalent cation, Mg2+ is most effective in catalyzing the dephosphorylation of pyridoxal 5’-phosphate Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31512
-
 x * 31512, sequence calculation Mus musculus
31698
-
 2 * 31698, sequence calculation, 2 * 32000, recombinant enzyme, SDS-PAGE Homo sapiens
32000
-
 2 * 31698, sequence calculation, 2 * 32000, recombinant enzyme, SDS-PAGE Homo sapiens
60000
-
 gel filtration, recombinant enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens vitamin B6 metabolism, catabolism of pyridoxal 5’-phosphate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96GD0
-
-
Mus musculus P60487
-
-

Purification (Commentary)

Purification (Comment) Organism
9.6fold, recombinant enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
brain highly abundant in, most highly represented in all the regions of central nerve system except the spinal cord Homo sapiens
-
liver major expression site additional to brain and testis Homo sapiens
-
additional information mRNA is differentially expressed in a tissue-specific manner, tissue distribution Homo sapiens
-
testis major expression site additional to brain and liver Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4
-
 pH 7.4, 37°C, hydrolysis of pyridoxal 5’-phosphate, recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information vitamin B6 metabolism, catabolism of pyridoxal 5’-phosphate Homo sapiens ?
-
 ?
additional information very low activity with with p-nitrophenyl phosphate Homo sapiens ?
-
 ?
pyridoxal 5'-phosphate + H2O
-
 Mus musculus pyridoxal + phosphate
-
 ?
pyridoxal 5'-phosphate + H2O highest specificity constant followed by pyridoxine 5’-phosphate Homo sapiens pyridoxal + phosphate
-
 ?
pyridoxamine 5'-phosphate + H2O
-
 Homo sapiens pyridoxamine + phosphate
-
 ?
pyridoxine 5'-phosphate + H2O
-
 Mus musculus pyridoxine + phosphate
-
 ?
pyridoxine 5'-phosphate + H2O
-
 Homo sapiens pyridoxine + phosphate
-
 ?
pyridoxine 5'-phosphate + H2O second highest specificity constant after pyridoxal 5’-phosphate Homo sapiens pyridoxine + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 31512, sequence calculation Mus musculus
dimer 2 * 31698, sequence calculation, 2 * 32000, recombinant enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 pyridoxamine 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens
1.25
-
 pyridoxine 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens
1.52
-
 pyridoxal 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Mus musculus sequence calculation
-
 6
Homo sapiens sequence calculation
-
 6