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Literature summary for 3.1.3.74 extracted from

  • Gao, G.J.; Fonda, M.L.
    Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytes (1994), J. Biol. Chem., 269, 8234-8239.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
2,2'-dithiodipyridine 0.005 mM, 50% inhibition Homo sapiens
4,4'-dithiodipyridine 0.005 mM, 50% inhibition Homo sapiens
5,5'-dithiobis(2-nitrobenzoic acid) 50 nM, 50% inhibition, incorporation of 1 mol per mol of subunit leads to complete inactivation, phosphate or dithiothreitol protects Homo sapiens
disulfide reagent reactivation by excess dithiothreitol, inactivation is due to formation of a mixed disulfide between the reagent and a free cysteinyl residue at or near the active site of enzyme Homo sapiens
iodoacetate 0.075 mM, 50% inhibition, incorporation of 0.6 mol per mol of subunit leads to complete inactivation, phosphate protects, inhibition kinetics Homo sapiens
additional information not inhibited by iodoacetamide or cystamine Homo sapiens
N-ethylmaleimide 0.1 mM, 50% inhibition, incorporation of 0.6 mol per mol of subunit leads to complete inactivation, inhibition kinetics, phosphate protects Homo sapiens
p-chloromercuribenzoate 250 nM, 50% inhibition Homo sapiens
phosphate competitive inhibitor Homo sapiens
thiol-specific reagent a variety of thiol-specific reagents inactivate in a time- and concentration-dependent manner, pyridoxal phosphate or phosphate protects Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000 Homo sapiens
64000
-
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte outdated packed erythrocytes Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxal 5'-phosphate + H2O best substrate, a cysteinyl residue at or near the active site is essential for activity, there may be only one free Cys per subunit Homo sapiens pyridoxal + phosphate
-
 ?
pyridoxine 5'-phosphate + H2O
-
 Homo sapiens pyridoxine + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000 Homo sapiens

Synonyms

Synonyms Comment Organism
pyridoxal-specific phosphatase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Homo sapiens