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Literature summary for 3.1.3.74 extracted from

  • Fonda, M.L.
    Purification and characterization of vitamin B6-phosphate phosphatase from human erythrocytes (1992), J. Biol. Chem., 267, 15978-15983.
    View publication on PubMed
Search for more literature for 3.1.3.74

General Stability

General Stability Organism
purified enzyme is unstable at low protein concentration, 0.002% Triton X-100 stabilizes, enzyme is unstable to freezing in the absence of glycerol Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-pyridoxic acid 5'-phosphate very effective inhibitor, 0.02 mM, 50% inhibition of pyridoxine 5’-phosphate hydrolysis Homo sapiens
EDTA 0.2 mM, complete inhibition in the absence of Mg2+, 50% inhibition in the presence of 1 mM Mg2+ Homo sapiens
fluoride 2 mM, 50% inhibition Homo sapiens
iodoacetate enzyme is very sensitive to Homo sapiens
molybdate very effective inhibitor, 0.0029 mM, 50% inhibition Homo sapiens
additional information not inhibited by nucleotide phosphates, phosphoamino acids, levamisole, L-phenylalanine, L(+)-tartrate, 5 mM ATP, 5 mM phosphoglycolate, 0.5 mM alpha- or beta-glycerophosphate, 0.5 mM 3-phosphoglycerate, 0.5 mM 2,3-bisphosphoglycerate, 5 mM pyridoxine, 5 mM pyridoxamine, 5 mM 4-pyridoxic acid, 5 mM 4-pyridine-carboxaldehyde, 5 mM isonicotinate, 5 mM 3-hydroxypyridine, 5 mM salicylaldehyde, 5 mM benzaldehyde Homo sapiens
N-ethylmaleimide enzyme is very sensitive to Homo sapiens
p-chloromercuribenzoate enzyme is very sensitive to Homo sapiens
p-nitrophenyl phosphate poor, 4 mM, 50% inhibition of pyridoxine 5’-phosphate hydrolysis Homo sapiens
Phenyl phosphate very poor inhibitor of pyridoxine 5’-phosphate hydrolysis Homo sapiens
phosphate competitive inhibitor with respect to pyridoxine 5’-phosphate, product inhibition Homo sapiens
pyridoxal weak, 11 mM, 50% inhibition of hydrolysis of pyridoxal 5’-phosphate or pyridoxine 5’-phosphate Homo sapiens
pyridoxal 5'-phosphate very effective inhibitor, 0.03 mM, 50% inhibition of pyridoxine 5’-phosphate hydrolysis Homo sapiens
pyridoxamine 5'-phosphate 0.5 mM, 50% inhibition of pyridoxine 5’-phosphate hydrolysis, less effective than pyridoxal 5’-phosphate or 4-pyridoxic acid 5’-phosphate Homo sapiens
Zn2+ very potent inhibitor, 50% inhibition in the presence of MgCl2 by 0.01 mM ZnCl2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00147
-
 pyridoxal 5'-phosphate pH 7.4, 37°C Homo sapiens
0.00255
-
 4-pyridoxic acid 5'-phosphate pH 7.4, 37°C Homo sapiens
0.00519
-
 pyridoxine 5'-phosphate pH 7.4, 37°C Homo sapiens
0.034
-
 pyridoxamine 5'-phosphate pH 7.4, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 enzyme has 17% as much activity with 1 mM CaCl2 than with MgCl2 Homo sapiens
Cu2+ enzyme has 4% as much activity with 1 mM Cu2+ than with MgCl2 Homo sapiens
Mg2+ MgCl2, required for activity, best activator Homo sapiens
Mn2+ enzyme has 2% as much activity with 1 mM Mn2+ than with MgCl2 Homo sapiens
additional information not activated by 100 mM NaCl, KCl, NaBr, NaI, NaNO3 or sodium acetate Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 2 * 33000, SDS-PAGE Homo sapiens
64000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens vitamin B6 metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
51000fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
0.575
-
 pH 7.4, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-deoxypyridoxine 5'-phosphate + H2O
-
 Homo sapiens 4-deoxypyridoxine + phosphate
-
 ?
4-pyridoxic acid 5'-phosphate + H2O the catalytic efficiency decreases in the following order: pyridoxal 5’-phosphate, 4-pyridoxic acid 5’-phosphate, pyridoxine 5’-phosphate and pyridoxamine 5’-phosphate Homo sapiens 4-pyridoxic acid + phosphate
-
 ?
additional information vitamin B6 metabolism Homo sapiens ?
-
 ?
additional information specifically dephosphorylates vitamin B6-phosphates, not: phenylphosphate, nucleotide phosphates, such as ATP, ADP, AMP, cAMP, FMN, phosphoamino acids, such as phosphoserine, phosphothreonine, phosphotyrosine, phosphoglycolate Homo sapiens ?
-
 ?
p-nitrophenyl phosphate + H2O slow hydrolysis Homo sapiens p-nitrophenol + phosphate
-
 ?
pyridoxal 5'-phosphate + H2O the catalytic efficiency decreases in the following order: pyridoxal 5’-phosphate, 4-pyridoxic acid 5’-phosphate, pyridoxine 5’-phosphate and pyridoxamine 5’-phosphate Homo sapiens pyridoxal + phosphate
-
 ?
pyridoxamine 5'-phosphate + H2O the catalytic efficiency decreases in the following order: pyridoxal 5’-phosphate, 4-pyridoxic acid 5’-phosphate, pyridoxine 5’-phosphate and pyridoxamine 5’-phosphate Homo sapiens pyridoxamine + phosphate
-
 ?
pyridoxine 5'-phosphate + H2O the catalytic efficiency decreases in the following order: pyridoxal 5’-phosphate, 4-pyridoxic acid 5’-phosphate, pyridoxine 5’-phosphate and pyridoxamine 5’-phosphate Homo sapiens pyridoxine + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.8
-
 phosphate pH 7.4, 37°C Homo sapiens