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Literature summary for 3.1.3.73 extracted from
- Structural and functional characterisation of an RNase HI domain from the bifunctional protein Rv2228c from Mycobacterium tuberculosis (2010), J. Bacteriol., 192, 2878-2886.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| maltose binding protein fusion protein expressed in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| N-terminal domain (RNase activity), sitting drop method | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.07 | - |
alpha-ribazole 5'-phosphate | - |
Mycobacterium tuberculosis |  |
| 5.5 | - |
p-nitrophenol phosphate | full length protein | Mycobacterium tuberculosis | |
| 13.6 | - |
p-nitrophenol phosphate | maltose binding protein fusion protein | Mycobacterium tuberculosis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WLH5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amylose affinity chromatography, gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-ribazole 5'-phosphate + H2O | - |
Mycobacterium tuberculosis | alpha-ribazole + phosphate | - |
? | |
| alpha-ribazole 5'-phosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | alpha-ribazole + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Mycobacterium tuberculosis | p-nitrophenol + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | p-nitrophenol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-ribazole phosphatase | bifunctional enzyme: RNase H activity | Mycobacterium tuberculosis |
| CobC | - |
Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
substrate p-nitrophenyl phosphate | Mycobacterium tuberculosis |
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