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Literature summary for 3.1.3.72 extracted from

  • Jog, S.P.; Garchow, B.G.; Mehta, B.D.; Murthy, P.P.N.
    Alkaline phytase from lily pollen: Investigation of biochemical properties (2005), Arch. Biochem. Biophys., 440, 133-140.
    View publication on PubMed
Search for more literature for 3.1.3.72

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Lilium longiflorum
MgCl2 0.166 M Lilium longiflorum
MgSO4 0.5 M Lilium longiflorum
vanadate 0.25-3 mM, 78-83% inhibition Lilium longiflorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate 37°C, pH 8.0 Lilium longiflorum
0.372
-
 p-nitrophenyl phosphate 37°C, pH 7.0 Lilium longiflorum

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2SO4 0.166 M, enhances activity Lilium longiflorum
Ca2+ activates, enzyme retains low level of activity even in Ca2+-free medium Lilium longiflorum
K2SO4 0.166 M, enhances activity Lilium longiflorum
KCl 0.5 M, activates Lilium longiflorum
Na2SO4 0.166 M, enhances activity Lilium longiflorum
NaCl 0.5 M, activates Lilium longiflorum
NH4Cl 0.5 M, enhances activity Lilium longiflorum

Organism

Organism UniProt Comment Textmining
Lilium longiflorum
-
 cv. Nellie White
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lilium longiflorum

Source Tissue

Source Tissue Comment Organism Textmining
pollen
-
 Lilium longiflorum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Lilium longiflorum p-nitrophenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
alkaline phytase
-
 Lilium longiflorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Lilium longiflorum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, stable Lilium longiflorum
55
-
 10 min, 60% loss of activity in assay containing CaCl2, compared to 40% in absence of CaCl2 Lilium longiflorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 activity with p-nitrophenyl phosphate Lilium longiflorum
8
-
 activity with myo-inositol-1,2,3,4,5,6-hexakisphosphate Lilium longiflorum