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Literature summary for 3.1.3.72 extracted from

  • Barrientos, L.; Scott, J.J.; Murthy, P.P.N.
    Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen (1994), Plant Physiol., 106, 1489-1495.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.72

General Stability

General Stability Organism
unstable, large loss of activity during the purification procedures Lilium longiflorum

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by 10 mM flouride Lilium longiflorum
phosphate inhibition by the phosphate released from enzyme Lilium longiflorum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Lilium longiflorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Lilium longiflorum phytic acid is a major component of seeds and pollen grains, localized in membrane-bound phytic-rich granules myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Lilium longiflorum
-
 cv. Nellie White
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lilium longiflorum

Source Tissue

Source Tissue Comment Organism Textmining
pollen
-
 Lilium longiflorum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.006
-
 in crude extracts Lilium longiflorum
0.019
-
 in DEAE-purified preparations Lilium longiflorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information two subsequent dephosphorylations occur adjacent to the D-5 hydroxyl group to yield myo-inositol-1,2,3-trikisphosphate as the final product, rates of removal of the second and third phosphates are significantly lower, possibly due to reduced specificity for IP5 and IP4 and inhibition by the phosphate released, IP4: only one isomer, either I-1,2,3,4-P4 or its enantiomer I-1,2,3,6-P4, no further hydrolysis of IP3 Lilium longiflorum ?
-
 ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O hydrolysis of the phosphate ester at the D-5 position Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate symmetrical product, only isomer of IP5 as product ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O high substrate specificity for phytic acid Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate symmetrical product, only isomer of IP5 as product ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O myo-inositol-1,2,3,4,5,6-hexakisphosphate is identical with phytic acid Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate symmetrical product, only isomer of IP5 as product ?
myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O phytic acid is a major component of seeds and pollen grains, localized in membrane-bound phytic-rich granules Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
alkaline phytase
-
 Lilium longiflorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Lilium longiflorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Lilium longiflorum