Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21 (DE3) | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting drop vapour diffusion technique, 10 mg/ml in 20 mM MES-NaOH, pH 6.35, 5 mM DTT, 1 mM EDTA, 5 mM MgCl2, and 760 mM NaCl, mixed with crystallization solution containing 0.02 M of each of the carboxylic acids, i.e. Na-formate, NH4-acetate, Na3-citrate, NaK-L-tartrate and Na-oxamate, 0.1 M MES/imidazole, pH 6.5, in a 1:1 molar ratio forming 0.002 ml drops, and a 30% v/v precipitant mixture containing 20% v/v ethylene glycol and 10% v/v PEG 8000, 20°C, 5 days, X-ray diffraction structure determination and analysis at 1.90 A resolution | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28200 | - |
2 * 28200, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
56400 | - |
recombinant enzyme, gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72K29 | - |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72K29 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21 (DE3) by anion exchange and cation exchange chromatography | Thermus thermophilus |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28200, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the haloalkanoic acid dehalogenase superfamily | Thermus thermophilus |
MPGP | - |
Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
assay at | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
metabolism | the synthesis of MG via mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase, the so-called two-step pathway, is the most prevalent route among these organisms. The phosphorylated intermediate mannosyl-3-phosphoglycerate is synthesized by the first enzyme and is subsequently dephosphorylated by the second | Thermus thermophilus |