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Literature summary for 3.1.3.70 extracted from

  • Empadinhas, N.; Marugg, J.D.; Borges, N.; Santos, H.; Da Costa, M.S.
    Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii. Biochemical and genetic characterization of key enzymes (2001), J. Biol. Chem., 276, 43580-43588.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.134
-
 2(alpha-D-mannosyl)-3-phosphoglycerate
-
 Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity without Mg2+ is 58% of the activity with 15 mM Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2(alpha-D-mannosyl)-3-phosphoglycerate + H2O Pyrococcus horikoshii enzyme is involved in biosynthesis of alpha-mannosylglycerate 2(alpha-D-mannosyl)-D-glycerate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2(alpha-D-mannosyl)-3-phosphoglycerate + H2O enzyme is highly specific for D-mannosyl-3-phosphoglycerate Pyrococcus horikoshii 2(alpha-D-mannosyl)-D-glycerate + phosphate
-
 ?
2(alpha-D-mannosyl)-3-phosphoglycerate + H2O enzyme is involved in biosynthesis of alpha-mannosylglycerate Pyrococcus horikoshii 2(alpha-D-mannosyl)-D-glycerate + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95 100
-
 Pyrococcus horikoshii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 108 70°C: about 45% of maximal activity, 108°C: about 20% of maximal activity Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
98
-
 half-life: 15.6 min Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2 6.4
-
 Pyrococcus horikoshii

pH Range

pH Minimum pH Maximum Comment Organism
4.2 7.5 pH 4.2: 60% of maximal actuvity, pH 7.5: about 45% of maximal activity Pyrococcus horikoshii