Literature summary for 3.1.3.7 extracted from

Lu, S.Y.; Zhao, G.R.; Wu, A.M.; Jenks, M.A.; Zhang, S.; Liu, J.Y.
Molecular cloning of a cotton phosphatase gene and its functional characterization (2010), Biochemistry (Moscow), 75, 85-94.

Search for more literature for 3.1.3.7

Application

Application	Comment	Organism
biotechnology	GhHL1 is a functional and good candidate gene that might be used to improve salt tolerance in plants	Gossypium hirsutum

Cloned(Commentary)

Cloned (Comment)	Organism
GhHL1 gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21, overexpression of GhHL1 complements yeast hal2 mutant and enhances yeast growth under elevated NaCl or LiCl	Gossypium hirsutum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on, optimal at 2 mM	Gossypium hirsutum
additional information	GhHL1 is Mg2+-dependent and cation-sensitive	Gossypium hirsutum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	x * 37000, recombinant enzyme, SDS-PAGE	Gossypium hirsutum

Organism

Organism	UniProt	Comment	Textmining
Gossypium hirsutum	Q8VWZ6	-	-
Gossypium hirsutum CRI 12	Q8VWZ6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
adenosine 3',5'-bisphosphate + H2O	-	Gossypium hirsutum	adenosine 5'-phosphate + phosphate	-	?
adenosine 3',5'-bisphosphate + H2O	-	Gossypium hirsutum CRI 12	adenosine 5'-phosphate + phosphate	-	?
adenosine 3',5'-bisphosphate sulfate + H2O	-	Gossypium hirsutum	adenosine 5'-phosphate sulfate + phosphate	-	?
adenosine 3',5'-bisphosphate sulfate + H2O	-	Gossypium hirsutum CRI 12	adenosine 5'-phosphate sulfate + phosphate	-	?
additional information	GhHL1 protein efficiently hydrolyzes 3'(2'),5'-bisphosphate nucleotide and 3'(2'),5'-bisphosphate nucleotide sulfate, at 128% of 3'(2'),5'-bisphosphate nucleotide levels, but has low or no activity with other tested compounds including 3'-AMP, 5'-AMP, or ADP. Purified recombinant GhHL1 protein dephosphorylates both 3',5'-bisphosphate nucleotide and inositol 1,4-bisphosphate, demonstrating dual 3',5'-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities, substrate specificity, overview	Gossypium hirsutum	?	-	?
additional information	GhHL1 protein efficiently hydrolyzes 3'(2'),5'-bisphosphate nucleotide and 3'(2'),5'-bisphosphate nucleotide sulfate, at 128% of 3'(2'),5'-bisphosphate nucleotide levels, but has low or no activity with other tested compounds including 3'-AMP, 5'-AMP, or ADP. Purified recombinant GhHL1 protein dephosphorylates both 3',5'-bisphosphate nucleotide and inositol 1,4-bisphosphate, demonstrating dual 3',5'-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities, substrate specificity, overview	Gossypium hirsutum CRI 12	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, recombinant enzyme, SDS-PAGE	Gossypium hirsutum

Synonyms

Synonyms	Comment	Organism
GhHL1	-	Gossypium hirsutum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Gossypium hirsutum

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	75	highest activity at 30-65°C, low activity at below 10°C, and 28.9% of maximal activity at 75°C	Gossypium hirsutum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Gossypium hirsutum

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	9	activity range, highest activity in the pH range from pH 7.0 to pH 8.5	Gossypium hirsutum

General Information

General Information	Comment	Organism
physiological function	overexpression of GhHL1 complements yeast hal2 mutant and enhances yeast growth under elevated NaCl or LiCl, showing a role in salt tolerance associated with ionic stress response	Gossypium hirsutum

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Release 2023.1 (January 2023)