Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
2-deoxy-D-glucose 6-phosphate | isoenzyme DogR1p | Saccharomyces cerevisiae | |
41 | - |
2-deoxy-D-glucose 6-phosphate | isoenzyme DogR2p | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, maximal activation with 10 mM | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
the enzyme is also present in wild type yeast, although its level is ten to twenty times lower than those found in the mutant | - |
Purification (Comment) | Organism |
---|---|
2 isoenzymes: DogR1p and DogR2p | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Deoxy-D-glucose 6-phosphate + H2O | - |
Saccharomyces cerevisiae | 2-Deoxy-D-glucose + phosphate | - |
? | |
2-Deoxyribose 5-phosphate + H2O | isoenzyme DogR1p: at 21% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 1% of the activity with 2-deoxy-D-glucose 6-phosphate | Saccharomyces cerevisiae | 2-Deoxyribose + phosphate | - |
? | |
Arabinose 5-phosphate + H2O | isoenzyme DogR1p: at 6% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 6% of the activity with 2-deoxy-D-glucose 6-phosphate | Saccharomyces cerevisiae | Arabinose + phosphate | - |
? | |
D-fructose 6-phosphate + H2O | isoenzyme DogR1p: at 2% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 17% of the activity with 2-deoxy-D-glucose 6-phosphate | Saccharomyces cerevisiae | D-fructose + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | isoenzyme DogR1p: at 42% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 13% of the activity with 2-deoxy-D-glucose 6-phosphate | Saccharomyces cerevisiae | D-ribose + phosphate | - |
? | |
Fructose 1-phosphate + H2O | isoenzyme DogR1p: at 12% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 106% of the activity with 2-deoxy-D-glucose 6-phosphate | Saccharomyces cerevisiae | Fructose + phosphate | - |
? | |
Glucose 6-phosphate + H2O | isoenzyme DogR1p: at 20% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 30% of the activity with 2-deoxy-D-glucose 6-phosphate | Saccharomyces cerevisiae | Glucose + phosphate | - |
? | |
Ribulose 5-phosphate + H2O | isoenzyme DogR1p: at 7% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 1% of the activity with 2-deoxy-D-glucose 6-phosphate | Saccharomyces cerevisiae | Ribulose + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
isoenzyme DogR1p | Saccharomyces cerevisiae |
40 | - |
isoenzyme DogR2p | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
isoenzyme DogR1p and DogR2p | Saccharomyces cerevisiae |