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Literature summary for 3.1.3.6 extracted from
- Purification and characterization of nucleases from tea leaves (1982), Agric. Biol. Chem., 46, 1261-1269.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Camellia sinensis |  |
| Cu2+ | - |
Camellia sinensis | |
| Fe3+ | - |
Camellia sinensis | |
| Mg2+ | - |
Camellia sinensis | |
| Mn2+ | - |
Camellia sinensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camellia sinensis | - |
nuclease A and nuclease B, both with 3'-nucleotidase activity | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nuclease A and nuclease B, both with 3'-nucleotidase activity | Camellia sinensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Camellia sinensis | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.04 M sodium acetate buffer, pH 5.5, 4 months stable | Camellia sinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-AMP + H2O | - |
Camellia sinensis | adenosine + phosphate | - |
? | |
| 3'-CMP + H2O | - |
Camellia sinensis | cytosine + phosphate | - |
? | |
| 3'-dAMP + H2O | - |
Camellia sinensis | 3'-deoxyadenosine + phosphate | - |
? | |
| 3'-GMP + H2O | - |
Camellia sinensis | guanosine + phosphate | - |
? | |
| 3'-UMP + H2O | - |
Camellia sinensis | uridine + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | nuclease B with 3'-nucleotidase activity | Camellia sinensis |
| 70 | - |
nuclease A with 3'-nucleotidase activity | Camellia sinensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable below | Camellia sinensis |
| 80 | - |
15 min, 80% inactivation | Camellia sinensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
substrates 3'-GMP, 3'-CMP | Camellia sinensis |
| 6.5 | - |
substrates 3'-UMP, 3'-AMP | Camellia sinensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | nuclease A and nuclease B, both with 3'-nucleotidase activity | Camellia sinensis |
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