Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium tetrathiomolybdate | 40% inhibition at 1 mM | Leishmania amazonensis | |
Cd2+ | - |
Leishmania amazonensis | |
Cu2+ | over 95% uinhibition at 1 mM | Leishmania amazonensis | |
molybdate | - |
Leishmania amazonensis | |
vanadate | 30% inhibition at 1 mM | Leishmania amazonensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Leishmania amazonensis | |
0.088 | - |
3'-AMP | pH 7.4, 25°C | Leishmania amazonensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | the 3'-nucleotidase activity is exclusively located on the Leishmania amazonensis external surface | Leishmania amazonensis | 9986 | - |
membrane | - |
Leishmania amazonensis | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania amazonensis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania amazonensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-AMP + H2O | - |
Leishmania amazonensis | adenosine + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3'NT/NU | - |
Leishmania amazonensis |
ecto-3'-nucleotidase | - |
Leishmania amazonensis |
ecto-3'-nucleotidase/nuclease | - |
Leishmania amazonensis |
La3'-nucleotidase | - |
Leishmania amazonensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Leishmania amazonensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Leishmania amazonensis |
General Information | Comment | Organism |
---|---|---|
additional information | ecto-3'-nucleotidase activity is 60fold higher than that of ecto-5'-nucleotidase in Leishmania amazonensis. Additionally, ecto-3'-nucleotidase activity is 2fold higher in virulent Leishmania amazonensis cells than in avirulent ones | Leishmania amazonensis |
physiological function | La3'-nucleotidase plays a significant role in the generation of adenosine, which contribute to mammalian host immune response impairment and establishment of infection, possible involvement of ecto-3'-nucleotidase in cellular adhesion | Leishmania amazonensis |