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Literature summary for 3.1.3.6 extracted from

  • Hatakeyama, T.; Mizuno, K.; Kurosawa, S.
    Purification and properties of a nuclease from the fruit body of Tricholoma matsutake (2010), Biosci. Biotechnol. Biochem., 74, 206-208.
    View publication on PubMed
Search for more literature for 3.1.3.6

General Stability

General Stability Organism
0.1 M NaCl, purified enzyme, 58% reduced activity after 30 min at 37°C Tricholoma matsutake

Inhibitors

Inhibitors Comment Organism Structure
GTP 66% inhibition at pH 8.0 and 1 0.1 mM Tricholoma matsutake

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl at 0.1 M, purified enzyme, 58% reduced activity after 30 min at 37°C Tricholoma matsutake

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 gel filtration Tricholoma matsutake

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-AMP + H2O Tricholoma matsutake
-
 adenosine + phosphate
-
 ?
additional information Tricholoma matsutake the enzyme cleaves RNA and heat-denatured DNA endonucleolytically producing 5'-mononucleotides and shows 3'-nucleotidase activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tricholoma matsutake
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from fruit bodies by ammonium sulfate fractionation, and further 12fold by anion exchange and cation exchange chromatography, hydroxyapatite chromatography, and gel filtration to homogeneity Tricholoma matsutake

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Tricholoma matsutake
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7900
-
 purified enzyme, pH 8.0, 37°C Tricholoma matsutake

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O
-
 Tricholoma matsutake adenosine + phosphate
-
 ?
additional information the enzyme cleaves RNA and heat-denatured DNA endonucleolytically producing 5'-mononucleotides and shows 3'-nucleotidase activity Tricholoma matsutake ?
-
 ?
poly(I) + H2O low activity Tricholoma matsutake ?
-
 ?
polyadenylic acid + H2O
-
 Tricholoma matsutake ?
-
 ?
polycytidylic acid + H2O low activity Tricholoma matsutake ?
-
 ?
polyuridylic acid + H2O
-
 Tricholoma matsutake ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Tricholoma matsutake

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Tricholoma matsutake

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 activity range, profile, overview Tricholoma matsutake

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 60 purified enzyme, 20 min, stable, sharp drop in activity above 60°C, profile, overview Tricholoma matsutake

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Tricholoma matsutake

pH Range

pH Minimum pH Maximum Comment Organism
5 9 activity range, profile, overview Tricholoma matsutake

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 purified enzyme, 20 min, 30°C, stable, 50% of maximal activity at pH 4.5 and pH 6.5, inactive below pH 4.0 and above pH 7.0, profile, overview Tricholoma matsutake