Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21(DE3), expressed in Saccharomyces cerevisiae JRM4 | Gossypium hirsutum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Gossypium hirsutum | |
K+ | - |
Gossypium hirsutum | |
lithium ion | - |
Gossypium hirsutum | |
Na+ | - |
Gossypium hirsutum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
3'-phosphoadenosine 5'-phosphosulfate | KM value lower than 0.01 mM | Gossypium hirsutum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal concentration 2mM, 3',5'-bisphosphate nucleotide as substrate | Gossypium hirsutum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Gossypium hirsutum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gossypium hirsutum | Q8VWZ6 | cv. CRI 121 | - |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Gossypium hirsutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenosine 5'-phosphosulfate + H2O | - |
Gossypium hirsutum | ? | - |
? | |
3'-phosphoadenosine 5'-phosphosulfate + H2O | - |
Gossypium hirsutum CRI 12 | ? | - |
? | |
3'-phosphoadenosine 5'-phosphosulfate + H2O | 128% of activity observed with 3'(2'),5'-bisphosphate nucleotide | Gossypium hirsutum | adenosine 5'-phosphosulfate + phosphate | - |
? | |
adenosine 3',5'-bisphosphate + H2O | - |
Gossypium hirsutum | adenosine 5'-phosphate + phosphate | - |
? | |
adenosine 3',5'-bisphosphate + H2O | - |
Gossypium hirsutum CRI 12 | adenosine 5'-phosphate + phosphate | - |
? | |
inositol 1,4,5-trisphosphate + H2O | 4.58% of activity observed with 3'(2'),5'-bisphosphate nucleotide | Gossypium hirsutum | ? + phosphate | - |
? | |
inositol 1,4-bisphosphate + H2O | 35% of activity observed with adenosine 3',5'-bisphosphate | Gossypium hirsutum | ? + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Gossypium hirsutum |
Synonyms | Comment | Organism |
---|---|---|
inositol polyphosphate 1-phosphatase | dual activities: 3',5'-bisphosphate nucleotidase | Gossypium hirsutum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | pH 7.5, 2 mM Mg2+, adenosine 3',5'-bisphosphate as substrate | Gossypium hirsutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
30°C, 2 mM Mg2+, adenosine 3',5'-bisphosphate as substrate | Gossypium hirsutum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | adenosine 3',5'-bisphosphate as substrate | Gossypium hirsutum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
30°C, pH 7.5, 0.55 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate | Gossypium hirsutum | Ca2+ | |
0.05 | - |
30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate | Gossypium hirsutum | Ca2+ | |
0.2 | - |
30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate | Gossypium hirsutum | lithium ion | |
0.8 | - |
30°C, pH 7.5, 5 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate | Gossypium hirsutum | Ca2+ | |
100 | - |
30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate | Gossypium hirsutum | Na+ | |
200 | - |
30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate | Gossypium hirsutum | K+ |