Literature summary for 3.1.3.56 extracted from

Hansen, C.A.; Johanson, R.A.; Williamson, M.T.; Williamson, J.R.
Purification and characterization of two types of soluble inositol phosphate 5-phosphomonoesterases from rat brain (1987), J. Biol. Chem., 262, 17319-17326.

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Inhibitors

Inhibitors	Comment	Organism	Structure
2,3-bisphosphoglycerate	-	Rattus norvegicus
ADP	-	Rattus norvegicus
AMP	slight inhibition	Rattus norvegicus
ATP	-	Rattus norvegicus
Ca2+	above 0.001 mM	Rattus norvegicus
D-fructose 1,6-bisphosphate	-	Rattus norvegicus
D-fructose 2,6-bisphosphate	-	Rattus norvegicus
D-glucose 6-phosphate	slight inhibition	Rattus norvegicus
EDTA	no activity in presence of 1 mM EDTA	Rattus norvegicus
Mg2+	above 0.5 mM	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0008	-	D-myo-Inositol 1,3,4,5-tetrakisphosphate	type I enzyme, pH 7.1, 30°C	Rattus norvegicus
0.003	-	D-myo-inositol 1,4,5-trisphosphate	type I enzyme, pH 7.1, 30°C	Rattus norvegicus
0.018	-	D-myo-inositol 1,4,5-trisphosphate	type II enzyme, pH 7.1, 30°C	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	two soluble forms, together both forms represent 26% of total activity	Rattus norvegicus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	moderate inhibition	Rattus norvegicus
Mg2+	required, maximum activity with 0.01 mM	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	gel filtration and SDS-PAGE, type I enzyme	Rattus norvegicus
160000	-	gel filtration and SDS-PAGE, type II enzyme	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O	Rattus norvegicus	-	D-myo-inositol 1,3,4-trisphosphate + phosphate	-	?
D-myo-inositol 1,4,5-trisphosphate + H2O	Rattus norvegicus	regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate	D-myo-inositol 1,4-bisphosphate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.06	-	purified type I enzyme, pH 7.1, 30°C	Rattus norvegicus
15.2	-	purified type II enzyme, pH 7.1, 30°C	Rattus norvegicus

Storage Stability

Storage Stability	Organism
-20°C, type II stable, type I lost 10% of activity per week	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O	-	Rattus norvegicus	D-myo-inositol 1,3,4-trisphosphate + phosphate	-	?
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O	substrate for type I but not type II enzyme	Rattus norvegicus	D-myo-inositol 1,3,4-trisphosphate + phosphate	-	?
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O	at 8% of the rate of inositol 1,4,5-trisphosphate hydrolysis	Rattus norvegicus	D-myo-inositol 1,3,4-trisphosphate + phosphate	-	?
D-myo-inositol 1,4,5-trisphosphate + H2O	-	Rattus norvegicus	D-myo-inositol 1,4-bisphosphate + phosphate	-	?
D-myo-inositol 1,4,5-trisphosphate + H2O	regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate	Rattus norvegicus	D-myo-inositol 1,4-bisphosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 60000, gel filtration, SDS-PAGE, type I, 1 * 160000, gel filtration, SDS-PAGE, type II	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	7	type II enzyme	Rattus norvegicus
7.5	-	type I enzyme	Rattus norvegicus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	9	-	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.07	-	Ca2+	-	Rattus norvegicus

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Release 2023.1 (January 2023)