Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Saccharomyces cerevisiae | |
Neocuproine | i.e. 2,9-dimethyl-1,10-phenanthroline, 0.1 mM Zn2+ stabilizes the activity | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuole | - |
Saccharomyces cerevisiae | 5773 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | dimer contains 1.3 mol copper per mol of subunit | Saccharomyces cerevisiae | |
Zn2+ | dimer contains 1.5 mol zinc per mol of subunit | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 2,6-bisphosphate + H2O | Saccharomyces cerevisiae | - |
D-fructose 2-phosphate + phosphate | - |
? | |
D-fructose 2,6-bisphosphate + H2O | Saccharomyces cerevisiae M1 | - |
D-fructose 2-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae M1 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
7000fold, strain M1 | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Saccharomyces cerevisiae | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Saccharomyces cerevisiae M1 | 4-nitrophenol + phosphate | - |
? | |
D-fructose 2,6-bisphosphate + H2O | - |
Saccharomyces cerevisiae | D-fructose 2-phosphate + phosphate | - |
? | |
D-fructose 2,6-bisphosphate + H2O | - |
Saccharomyces cerevisiae M1 | D-fructose 2-phosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7.7, about 60% loss of activity after 15 min, fructose-2,6-bisphosphate 6-phosphohydrolase activity | Saccharomyces cerevisiae |
40 | - |
pH 7.0, 5 min, stable, fructose-2,6-bisphosphate 6-phosphohydrolase activity | Saccharomyces cerevisiae |
50 | - |
pH 7.0, 5 min, about 30% loss of activity, fructose-2,6-bisphosphate 6-phosphohydrolase activity | Saccharomyces cerevisiae |
60 | - |
pH 7.0, 5 min, about 70% loss of activity, fructose-2,6-bisphosphate 6-phosphohydrolase activity | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | D-fructose 2,6-bisphosphate | Saccharomyces cerevisiae |
6 | - |
with D-fructose 2,6-bisphosphate as substrate | Saccharomyces cerevisiae |
9 | - |
above, 4-nitrophenyl phosphate | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | pH 4: about 25% of activity maximum, pH 7.5: about 20% of activity maximum, fructose 2,6-bisphosphate | Saccharomyces cerevisiae |