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Literature summary for 3.1.3.53 extracted from

  • Khasnis, M.; Nakatomi, A.; Gumpper, K.; Eto, M.
    Reconstituted human myosin light chain phosphatase reveals distinct roles of two inhibitory phosphorylation sites of the regulatory subunit, MYPT1 (2014), Biochemistry, 53, 2701-2709.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.53

Cloned(Commentary)

Cloned (Comment) Organism
MLCP complex (MYPT1-PP1 dimer) is transiently expressed in COS1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
DELTA495-1030 using a deletion mutant it is shown that the C-terminal domain of human MYPT1(495-1030) is responsible for the binding to the N-terminal portion of myosin light meromyosin Homo sapiens
T696A substitution of Thr696 with Ala eliminates the phosphorylation-dependent inhibition Homo sapiens
T853A the extent of inhibition of mutant T853A MLCP is indistinguishable from that of wild-type Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ at 1 mM inhibitory effect Homo sapiens
okadaic acid
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O14974
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein selective thiophosphorylation of MYPT1 at Thr696 with ROCK inhibits the MLCP activity 30%, whereas the Thr853 thio-phosphorylation does not alter the phosphatase activity. Both Thr696 and Thr853 sites undergo autodephosphorylation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
P-MLC20 + H2O P-MLC20: phospho-peptide mimicking MLC20(3-26:P-Ser19) Homo sapiens MLC20 + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens