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Literature summary for 3.1.3.53 extracted from
- Purification and characterization of the plasmodial phosphatase that hydrolyses the phosphorylated light chain of Physarum myosin II from Physarum polycephalum (2010), Cell Biol. Int., 34, 827-835.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibits at 0.01 mM | Physarum polycephalum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
[myosin light-chain] phosphate | pH 7.6, temperature not specified in the publication | Physarum polycephalum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates at 0.0085 mM | Physarum polycephalum | |
| Mn2+ | highly activating at 0.0016 mM | Physarum polycephalum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Physarum polycephalum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [myosin light-chain] phosphate + H2O | Physarum polycephalum | - |
[myosin light-chain] + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Physarum polycephalum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 340fold by ion exchange and hydrophobic interaction chromatography | Physarum polycephalum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.028 | - |
purified enzyme, pH 7.6, temperature not specified in the publication | Physarum polycephalum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [myosin light-chain] phosphate + H2O | - |
Physarum polycephalum | [myosin light-chain] + phosphate | - |
? | |
| [myosin light-chain] phosphate + H2O | substrate is phosphorylated Physarum myosin II | Physarum polycephalum | [myosin light-chain] + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| plasmodial phosphatase | - |
Physarum polycephalum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Physarum polycephalum |
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