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Literature summary for 3.1.3.53 extracted from

  • Hirano, K.; Phan, B.C.; Hartshorne, D.J.
    Interactions of the subunits of smooth muscle myosin phosphatase (1997), J. Biol. Chem., 272, 3683-3688.
    View publication on PubMed
Search for more literature for 3.1.3.53

Protein Variants

Protein Variants Comment Organism
additional information various truncation mutants of the 130 kDa subunit M130 Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibitory effect of several truncation mutants of the 130 kDa subunit M130 on the activity of the catalytic subunit PP1c, IC50 values Gallus gallus
additional information inhibitory effect of several truncation mutants of the 130 kDa subunit M130 on the activity of the catalytic subunit PP1c, IC50 values Meleagris gallopavo

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 x * 130000 + x * 38000 + x * 20000, 38 kDa catalytic subunit PP1c, 130 kDa and 20 kDa non-catalytic subunits M130 and M20, investigation of the interactions among PP1c, M20 and various mutants of M130 using the yeast two-hybrid system Gallus gallus
38000
-
 x * 130000 + x * 38000 + x * 20000, 38 kDa catalytic subunit PP1c, 130 kDa and 20 kDa non-catalytic subunits M130 and M20, investigation of the interactions among PP1c, M20 and various mutants of M130 using the yeast two-hybrid system Gallus gallus
130000
-
 x * 130000 + x * 38000 + x * 20000, 38 kDa catalytic subunit PP1c, 130 kDa and 20 kDa non-catalytic subunits M130 and M20, investigation of the interactions among PP1c, M20 and various mutants of M130 using the yeast two-hybrid system Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Meleagris gallopavo
-
 catalytic subunit PP1c
-

Source Tissue

Source Tissue Comment Organism Textmining
gizzard
-
 Gallus gallus
-
gizzard
-
 Meleagris gallopavo
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
 Meleagris gallopavo ?
-
 ?
additional information interactions among the 38 kDa catalytic subunit PP1c, the 130 kDa and the 20 kDa non-catalytic subunits M130 and M20 or fragments of them, M130 has 2 binding sites for PP1c: one site in the N-terminal 38 residues and a weaker site in the ankyrin repeats region, activation of phosphatase and binding of PP1c and substrate are properties of the N-terminal one-third of M130 Gallus gallus ?
-
 ?
myosin light-chain phosphate + H2O 38 kDa catalytic subunit PP1c and holoenzyme are active, the 130 kDa subunit M130 activates PP1c activity, effect of truncation mutants of M130 on PP1c activity Gallus gallus myosin light-chain + phosphate
-
 ?
myosin light-chain phosphate + H2O 38 kDa catalytic subunit PP1c and holoenzyme are active, the 130 kDa subunit M130 activates PP1c activity, effect of truncation mutants of M130 on PP1c activity Meleagris gallopavo myosin light-chain + phosphate
-
 ?
myosin light-chain phosphate + H2O 20 kDa myosin light chain Gallus gallus myosin light-chain + phosphate
-
 ?
myosin light-chain phosphate + H2O 20 kDa myosin light chain Meleagris gallopavo myosin light-chain + phosphate
-
 ?
phosphorylated phoshorylase A + H2O 38 kDa catalytic subunit PP1c Meleagris gallopavo phosphorylase A + phosphate
-
 ?

Subunits

Subunits Comment Organism
?
-
 Meleagris gallopavo
? x * 130000 + x * 38000 + x * 20000, 38 kDa catalytic subunit PP1c, 130 kDa and 20 kDa non-catalytic subunits M130 and M20, investigation of the interactions among PP1c, M20 and various mutants of M130 using the yeast two-hybrid system Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Gallus gallus
30
-
 assay at Meleagris gallopavo

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Meleagris gallopavo