Cloned (Comment) | Organism |
---|---|
cDNAs encoding the 133 kDa myosin-binding subunit and the 20 kDa regulatory subunit are cloned and expressed in Escherichia coli BL21(DE3) | Gallus gallus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutants of the 133 kDa myosin-binding subunit | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acidic phospholipid | interaction of enzyme with acidic phospholipids inhibits activity toward phosphorylated myosin | Gallus gallus | |
okadaic acid | potent inhibitor of PP-1 and PP-2A | Gallus gallus | |
phosphatidic acid | most effective inhibitor among acidic phospholipids, followed by phosphatidylserine and phosphatidylinositol, phosphorylated myosin as substrate | Gallus gallus | |
phosphatidylinositol | phosphatidic acid is the most effective inhibitor among acidic phospholipids, followed by phosphatidylserine and phosphatidylinositol, phosphorylated myosin as substrate | Gallus gallus | |
phosphatidylserine | phosphatidic acid is the most effective inhibitor among acidic phospholipids, followed by phosphatidylserine and phosphatidylinositol, phosphorylated myosin as substrate | Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | 133 kDa myosin-binding subunit | Rattus norvegicus | 5829 | - |
membrane | enzyme may interact with membranes, phosphorylation by protein kinase A may modify interaction, distribution of the 130/133 kDa myosin-binding subunit at the membrane | Gallus gallus | 16020 | - |
plasma membrane | 133 kDa myosin-binding subunit | Rattus norvegicus | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE | Gallus gallus |
38000 | - |
x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE | Gallus gallus |
130000 | - |
x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Rattus norvegicus |
phosphoprotein | 130/133 kDa myosin-binding subunits M130/M133 and 20 kDa regulatory subunit M20 are phosphorylated by protein kinase A to 3 and 1 mol phosphate/mol of subunit, phosphorylation of holoenzyme decreases phospholipid binding, M130/133: C-terminal phosphorylation site involved in regulation of phospholipid binding, no phosphorylation of the catalytic subunit PP-1cdelta, protein kinase C also phosphorylates M130/M133 and M20, but no effect on binding of phospholipids | Gallus gallus |
Purification (Comment) | Organism |
---|---|
native holoenzyme | Gallus gallus |
recombinant 133 kDa myosin-binding subunit and 20 kDa regulatory subunit, expressed in Escherichia coli | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gizzard | - |
Gallus gallus | - |
REF-52 cell | rat embryonic fibroblast cells, mitotic, 133 kDa myosin-binding subunit | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Rattus norvegicus | ? | - |
? | |
additional information | enzyme binds to vesicles of acidic phospholipids, i.e. phosphatidylserine, phosphatidylinositol and phosphatidic acid, but not to neutral phospholipids, phosphatidylserine binding decreases by increasing ionic strength and Mg2+ concentration, phospholipid binding is associated with the C-terminal part of the 130/133 kDa myosin-binding subunit and the 20 kDa regulatory subunit | Gallus gallus | ? | - |
? | |
myosin light-chain phosphate + H2O | - |
Gallus gallus | myosin light-chain + phosphate | - |
? | |
phosphorylated myosin + H2O | - |
Gallus gallus | myosin + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE | Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Gallus gallus |