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Literature summary for 3.1.3.5 extracted from

  • Mittal, R.; Das, J.; Sharma, C.B.
    Purification and characterization of 5'-nucleotidase from the Golgi apparatus of peanut cotyledons (1988), Plant Sci., 55, 93-101.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.91
-
5'-AMP
-
Arachis hypogaea

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
Arachis hypogaea 5794
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53700
-
x * 53700, enzyme from Golgi apparatus, SDS-PAGE Arachis hypogaea

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
peanut
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification 38,5% carbohydrate, enzyme of Golgi apparatus Arachis hypogaea

Purification (Commentary)

Purification (Comment) Organism
-
Arachis hypogaea

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Arachis hypogaea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
167.7
-
-
Arachis hypogaea

Storage Stability

Storage Stability Organism
-20°C, purified enzyme inactivated in 24 h, membrane-bound enzyme stable for 2 weeks Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O highly specific, no hydrolysis of other nucleotides Arachis hypogaea adenosine + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 53700, enzyme from Golgi apparatus, SDS-PAGE Arachis hypogaea

Synonyms

Synonyms Comment Organism
GA-AMPase
-
Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
Arachis hypogaea

pH Range

pH Minimum pH Maximum Comment Organism
5 6
-
Arachis hypogaea