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Literature summary for 3.1.3.5 extracted from

  • Ikura, Y.; Horikoshi, K.
    Manganese-dependent production, and properties, of 5'-nucleotidase by alkalophilic Bacillus No. A-59 (1989), Agric. Biol. Chem., 53, 645-649.
No PubMed abstract available
Search for more literature for 3.1.3.5

Inhibitors

Inhibitors Comment Organism Structure
B4O72-
-
 Bacillus sp. (in: Bacteria)
NaAsO2
-
 Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 No. A-59
-
Bacillus sp. (in: Bacteria) No. A-59
-
 No. A-59
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
57.3
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Bacillus sp. (in: Bacteria) adenosine + phosphate
-
 ?
5'-AMP + H2O
-
 Bacillus sp. (in: Bacteria) No. A-59 adenosine + phosphate
-
 ?
5'-CMP + H2O 23.1% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) cytidine + phosphate
-
 ?
5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Bacillus sp. (in: Bacteria) deoxyadenosine + phosphate
-
 ?
5'-dAMP + H2O 19.7% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) deoxyadenosine + phosphate
-
 ?
5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Bacillus sp. (in: Bacteria) No. A-59 deoxyadenosine + phosphate
-
 ?
5'-dAMP + H2O 19.7% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) No. A-59 deoxyadenosine + phosphate
-
 ?
5'-GMP + H2O 16.7% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) guanosine + phosphate
-
 ?
5'-GMP + H2O 16.7% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) No. A-59 guanosine + phosphate
-
 ?
5'-TMP + H2O 7.4% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) thymidine + phosphate
-
 ?
5'-UMP + H2O 10.6% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) uridine + phosphate
-
 ?
5'-UMP + H2O 10.6% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) No. A-59 uridine + phosphate
-
 ?
ADP + H2O 9.6% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) adenosine + diphosphate
-
 ?
cyclic 3',5'-AMP + H2O 8.8% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 15 min, 2% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5 9.5
-
 Bacillus sp. (in: Bacteria)