Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 89.5% residual activity at 5 mM | Macrovipera lebetina | |
Cu2+ | 3.9% residual activity at 5 mM | Macrovipera lebetina | |
EDTA | - |
Macrovipera lebetina | |
Fe3+ | 3.3% residual activity at 5 mM | Macrovipera lebetina | |
Mg2+ | 80.9% residual activity at 5 mM | Macrovipera lebetina | |
vanillic acid | - |
Macrovipera lebetina | |
Zn2+ | 2.1% residual activity at 5 mM | Macrovipera lebetina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the enzyme activity is enhanced by Mn2+ (170% activity at 5 mM) | Macrovipera lebetina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
gel filtration | Macrovipera lebetina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-AMP + H2O | Macrovipera lebetina | - |
adenosine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macrovipera lebetina | W8EFS0 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography, CM52 cellulose column chromatography, Superdex 200 gel filtration, and Sephadex G-100 gel filtration | Macrovipera lebetina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Macrovipera lebetina | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-AMP + H2O | - |
Macrovipera lebetina | adenosine + phosphate | - |
? | |
5'-AMP + H2O | best substrate | Macrovipera lebetina | adenosine + phosphate | - |
? | |
5'-GMP + H2O | - |
Macrovipera lebetina | guanosine + phosphate | - |
? | |
ADP + H2O | - |
Macrovipera lebetina | 5'-AMP + phosphate | - |
? | |
ATP + H2O | lowest activity | Macrovipera lebetina | ADP + phosphate | - |
? | |
additional information | no activity with 3'-AMP, 3',5'-cAMP, and 3',5'-cGMP | Macrovipera lebetina | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, SDS-PAGE | Macrovipera lebetina |
Synonyms | Comment | Organism |
---|---|---|
5'-NT | - |
Macrovipera lebetina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | the enzyme is stable from 10-37°C. The activity of the enzyme decreases after heating at 37°C, at 50°C approximately two third of the activity is left, at 60°C the activity is almost entirely lost | Macrovipera lebetina |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme strongly inhibits ADP- or collagen-induced platelet aggregation | Macrovipera lebetina |