Literature summary for 3.1.3.5 extracted from

Kozarski, M.; Kubacka, D.; Wojtczak, B.A.; Kasprzyk, R.; Baranowski, M.R.; Kowalska, J.
7-Methylguanosine monophosphate analogues with 5-(1,2,3-triazoyl) moiety Synthesis and evaluation as the inhibitors of cNIIIB nucleotidase (2018), Bioorg. Med. Chem., 26, 191-199 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) RIL cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
5'-deoxy-7-methyl-5'-(4-phosphono-1H-pyrazol-1-yl)guanosine	moderately potent inhibitor	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	5 mM used in assay conditions	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex 75 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-methylguanosine diphosphate + H2O	preferred substrate	Homo sapiens	7-methylguanosine monophosphate + phosphate	-	?
7-methylguanosine monophosphate + H2O	preferred substrate	Homo sapiens	7-methylguanosine + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
cNIIIB nucleotidase	-	Homo sapiens
cytosolic 5'-nucleotidase	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0878	-	at pH 7.5 and 30°C	Homo sapiens	5'-deoxy-7-methyl-5'-(4-phosphono-1H-pyrazol-1-yl)guanosine

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Release 2023.1 (January 2023)