Protein Variants | Comment | Organism |
---|---|---|
D351E | strong decrease in nucleotidase activity, strong reduction in affinity towards Mg2+ | Bos taurus |
D356E | strong decrease in nucleotidase activity, strong reduction in affinity towards Mg2+ | Bos taurus |
D356N | strong increase in Km-value for inosine | Bos taurus |
K292M | strong decrease in nucleotidase activity | Bos taurus |
K292R | strong decrease in nucleotidase activity | Bos taurus |
M53I | lowest nucleotidase vs. phosphotransferase activity ratio of all mutants tested | Bos taurus |
M53N | strong decrease in catalytic efficiency | Bos taurus |
S251A | kinetic behaviour almost similar to wild-type | Bos taurus |
S251T | kinetic behaviour almost similar to wild-type | Bos taurus |
T249S | kinetic behaviour almost similar to wild-type | Bos taurus |
T249V | strongly reduced enzyme activity | Bos taurus |
T56R | mutant devoid of phosphotransferase and nucleotidase activities | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
5'-IMP | wild-type | Bos taurus | |
0.1 | - |
5'-IMP | mutant M53N | Bos taurus | |
0.1 | - |
5'-IMP | mutant S251A | Bos taurus | |
0.2 | - |
5'-IMP | mutant D356E | Bos taurus | |
0.2 | - |
5'-IMP | mutant D356N | Bos taurus | |
0.2 | - |
5'-IMP | mutant T249S | Bos taurus | |
0.25 | - |
Inosine | mutant T249v | Bos taurus | |
0.3 | - |
5'-IMP | mutant S251T | Bos taurus | |
0.4 | - |
5'-IMP | mutant T249v | Bos taurus | |
0.5 | - |
Inosine | mutant K292M | Bos taurus | |
0.8 | - |
Inosine | mutant M53I | Bos taurus | |
0.9 | - |
Inosine | mutant D351E | Bos taurus | |
0.9 | - |
Inosine | mutant T249S | Bos taurus | |
1 | - |
Inosine | mutant D356E | Bos taurus | |
1 | - |
5'-IMP | mutant M53I | Bos taurus | |
1 | - |
Inosine | mutant S251A | Bos taurus | |
1.1 | - |
Inosine | wild-type | Bos taurus | |
1.1 | - |
Inosine | mutant M53N | Bos taurus | |
1.5 | - |
Inosine | mutant S251T | Bos taurus | |
10.2 | - |
Inosine | mutant D356N | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
isoform 5'-nucleotidase II | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 5'-ribonucleotide + H2O = a ribonucleoside + phosphate | conserved motif I is involved in formation and stabilization of the covalent enzyme-phosphate intermediate. T249 and K292 in motif II contribute to stabilize the enzyme-phospho adduct. D351 and D356 in motif III coordinate the magnesium ion required for catalysis | Bos taurus |
Renatured (Comment) | Organism |
---|---|
inhibition by CuCl2, 5,5-dithiobis-(2-nitro-benzoic acid) is reverted by presence of reducing agent | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-IMP + H2O | - |
Bos taurus | inosine + phosphate | - |
r | |
inosine + phosphate | - |
Bos taurus | 5'-IMP + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Inosine | mutant D351E | Bos taurus | |
0.1 | - |
Inosine | mutant K292M | Bos taurus | |
0.3 | - |
Inosine | mutant T249v | Bos taurus | |
0.6 | - |
Inosine | mutant M53N | Bos taurus | |
1.4 | - |
Inosine | mutant D356N | Bos taurus | |
2.2 | - |
Inosine | mutant D356E | Bos taurus | |
3.4 | - |
Inosine | mutant S251T | Bos taurus | |
3.7 | - |
Inosine | mutant M53I | Bos taurus | |
12.3 | - |
Inosine | mutant S251A | Bos taurus | |
19.8 | - |
Inosine | mutant T249S | Bos taurus | |
40 | - |
Inosine | wild-type | Bos taurus |