Protein Variants | Comment | Organism |
---|---|---|
D811E | site-directed mutagenesis, ectopic expression of the D811E mutant form of PTPN3 does not reduce phosphorylation of substrate peptide Eps15 | Homo sapiens |
H812F | site-directed mutagenesis, ectopic expression of the H812F mutant form of PTPN3 does not reduce phosphorylation of substrate peptide Eps15 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[a protein]-tyrosine phosphate + H2O | Homo sapiens | - |
[a protein]-tyrosine + phosphate | - |
? | |
[Eps15 peptide]-tyrosine phosphate + H2O | Homo sapiens | substrate is a phosphopeptide fragment of substrate epidermal growth factor receptor. Eps15 is a scaffolding adaptor that regulates endocytosis and trafficking of the EGFR and is a substrate for enzyme PTPN3 | [Eps15 peptide]-tyrosine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P26045 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[a protein]-tyrosine phosphate + H2O | - |
Homo sapiens | [a protein]-tyrosine + phosphate | - |
? | |
[Eps15 peptide]-tyrosine phosphate + H2O | substrate is a phosphopeptide fragment of substrate epidermal growth factor receptor. Eps15 is a scaffolding adaptor that regulates endocytosis and trafficking of the EGFR and is a substrate for enzyme PTPN3 | Homo sapiens | [Eps15 peptide]-tyrosine + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
protein tyrosine phosphatase | - |
Homo sapiens |
PTP | - |
Homo sapiens |
PTPN3 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | analysis of molecular basis of substrate specificity in the FERM-containing subfamily of nonreceptor PTPs, overview. These enzymes are characterized by the presence of an N-terminal FERM plasma-membrane-localization domain and a C-terminal catalytic domain | Homo sapiens |
additional information | determination of key interactions that likely determine both substrate recognition and the catalytic activity of the complex. e.g. an important interaction between H812 of the enzyme and a proline residue adjacent to the phosphotyrosine of the peptide substrate, which results in an atypical conformation of the C-terminal part of the peptide substrate. If the proline is replaced by valine, the atypical conformation is lost. Residues H812 and D811 of PTPN3 are not required to regulate Eps15-dependent EGFR signaling in cultured cells | Homo sapiens |
physiological function | protein tyrosine phosphatases (PTPs) play critical roles in cell signaling pathways. Together with the protein kinases, they control the balance of phosphorylated species, enabling specific and varied signaling responses | Homo sapiens |