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Literature summary for 3.1.3.48 extracted from

  • Singh, N.; Yadav, S.; Rathaur, S.
    Identification and characterization of novel membrane-bound PRL protein tyrosine phosphatases from Setaria cervi, a bovine filarial parasite (2015), Parasitol. Res., 114, 4267-4279 .
    View publication on PubMed
Search for more literature for 3.1.3.48

Activating Compound

Activating Compound Comment Organism Structure
DTT highly activating at 2 mM Setaria cervi

Inhibitors

Inhibitors Comment Organism Structure
1,2-Cyclohexanedione 40% inhibition at 1 mM Setaria cervi
1-ethyl-3-dimethylaminopropyl carbodiimide 90% inhibition at 25 mM Setaria cervi
4-hydroxymercuribenzoate 62% inhibition at 2.5 mM Setaria cervi
Acetylsalicylic acid 80% inhibition at 0.2 mM Setaria cervi
Ag+ 62% inhibition at 2.5 mM Setaria cervi
Ammonium molybdate 98% inhibition at 2 mM Setaria cervi
cantharidin 17% inhibition at 1 mM Setaria cervi
diethylcarbamazine 12% inhibition at 0.2 mM Setaria cervi
diethyldicarbonate 7% inhibition at 1 mM Setaria cervi
EDTA 51% inhibition at 5 mM Setaria cervi
iodoacetic acid complete inhibition at 1.0 mM Setaria cervi
L-(+)-Tartrate 6.5% inhibition at 5 mM Setaria cervi
okadaic acid 7.5% inhibition at 0.01 mM Setaria cervi
Phenylarsine oxide a specific PTP inhibitor, 79% inhibition at 1.0 nM Setaria cervi
SK7 a metal chalcone, complete inhibition at 0.02 mM Setaria cervi
Sodium fluoride 96% inhibition at 2 mM Setaria cervi
sodium orthovanadate 99.5% inhibition at 0.05 mM Setaria cervi
Zn2+ 65.5% inhibition at 2.5 mM Setaria cervi

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Setaria cervi 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates at 2.5 mM Setaria cervi
Mg2+ activates slightly at 2.5 mM Setaria cervi
Mn2+ activates at 2.5 mM Setaria cervi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 gel filtration Setaria cervi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[a protein]-tyrosine phosphate + H2O Setaria cervi
-
 [a protein]-tyrosine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Setaria cervi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from adult females by concanavalin A affinity chromatography and cation exchange chromatography to homogeneity Setaria cervi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O acid phosphatase activity, best tested substrate Setaria cervi 4-nitrophenol + phosphate
-
 ?
additional information substrate specificity of enzyme PRL, overview. The enzyme also hydrolyzes adenosine diphosphate, fructose 6-phosphate, sodium alpha-naphthyl phosphate, and phosphoenol pyruvate Setaria cervi ?
-
 ?
O-phospho-L-serine + H2O very low activity with Setaria cervi L-serine + phosphate
-
 ?
O-phospho-L-threonine + H2O
-
 Setaria cervi L-threonine + phosphate
-
 ?
O-phospho-L-tyrosine + H2O
-
 Setaria cervi L-tyrosine + phosphate
-
 ?
[a protein]-tyrosine phosphate + H2O
-
 Setaria cervi [a protein]-tyrosine + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 21000, SDS-PAGE, 1 * 20527, mass spectrometry Setaria cervi

Synonyms

Synonyms Comment Organism
acid phosphatase activity
-
 Setaria cervi
PRL
-
 Setaria cervi
protein tyrosine phosphatase
-
 Setaria cervi
PTP
-
 Setaria cervi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Setaria cervi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 80 activity range, profile overview, maximal activity at 37°C Setaria cervi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the enzyme is almost denatured at 60°C Setaria cervi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Setaria cervi

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8 activity range, profile overview, maximal activity at pH 5.5 Setaria cervi

Expression

Organism Comment Expression
Setaria cervi the enzyme is slightly upregulated at oxidative stress generated by a Fe2+/H2O2/ascorbate system up

General Information

General Information Comment Organism
additional information the purified enzyme seems to harbor Cys, His, Asp, and Arg residues in its active site Setaria cervi