Cloned (Comment) | Organism |
---|---|
gene PTPN6, recombinant expression of wild-type full-length enzyme and isolated catalytic enzyme domain SHP-1cat, and of V451M mutant full-length enzyme and isolated catalytic enzyme domain SHP-1cat in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
V451M | site-directed mutagenesis, the mutation lies in a conserved motif adjacent to the protein tyrosine phosphatase (PTP) consensus sequence and alters catalytic function. Mutant V451M possesses increased catalytic activity as compared to the wild-type enzyme. When assayed with 4-nitrophenyl phosphatase as substrate, mutant V451M shows higher activity regardless of the pNPP substrate concentration used. The mutant shows increased thermolability compared to wild-type | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[a protein]-tyrosine phosphate + H2O | Homo sapiens | - |
[a protein]-tyrosine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P29350 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
DADE(pY)LIPQQG + H2O | - |
Homo sapiens | DADEYLIPQQG + phosphate | - |
? | |
[a protein]-tyrosine phosphate + H2O | - |
Homo sapiens | [a protein]-tyrosine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | unlike the catalytic domain of SHP-1 (SHP-1cat), full-length SHP-1 is a multi-domain enzyme that contains two Src-homology 2 (SH2) domains in addition to its PTP domain | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
protein tyrosine phosphatase | - |
Homo sapiens |
PTP | - |
Homo sapiens |
PTPN6 | - |
Homo sapiens |
SHP-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
above 42°C, the activity of the mutant proteins decreases substantially, dropping off to only a fraction of that of the corresponding wild-type enzymes | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | unlike the catalytic domain of SHP-1 (SHP-1cat), full-length SHP-1 is a multi-domain enzyme that contains two Src-homology 2 (SH2) domains in addition to its PTP domain | Homo sapiens |
physiological function | in human cells, enzyme SHP-1's phosphatase activity is regulated through an autoinhibitory interaction between its catalytic PTP domain and one of its SH2 domains, in the autoinhibited state of SHP-1, the amino-terminal SH2 domain blocks the PTP-domain's active site | Homo sapiens |