Cloned (Comment) | Organism |
---|---|
overexpression of PTP1B in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of stable cell lines expressing a short hairpin RNA targeting the phosphatase transcript and reduction of PTP1B expression levels down to 20%. Upon PTP1B overexpression, phosphorylation of PLC-gamma1 and Gab1 sharply decreases when compared with controls where PLC-gamma1 and Gab1 phosphorylations | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gab1 tyrosine phosphate + H2O | - |
Homo sapiens | Gab1 tyrosine + phosphate | - |
? | |
additional information | substrate specificity analysis using a trapping mutant of the phosphatase, which is a point mutation that maintains specificity but does not allow detachment of the phosphate, overview. The enzyme is also active on 4-nitrophenyl phosphate, but is not active on GRB2 | Homo sapiens | ? | - |
? | |
PLC-gamma1 tyrosine phosphate + H2O | - |
Homo sapiens | PLC-gamma1 tyrosine + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PTP1B | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | phosphatases interact only transiently with their substrates | Homo sapiens |
physiological function | PTP1B-mediated dephosphorylation of Gab1 negatively affects its EGF-induced association with the phosphatase SHP2. The dissociation of this signaling complex is accompanied by a decrease of ERK MAP kinase phosphorylation and activation. PTP1B specifically interferes with SHP2 recruitment by Gab1 and with ERK1/2 activation | Homo sapiens |