Literature summary for 3.1.3.47 extracted from

  • Gil G.; Sitges M.; Hegardt F.G.
    Purification and properties of rat liver hydroxymethylglutaryl CoA reductase phosphatases (1981), Biochim. Biophys. Acta, 663, 211-221.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
fluoride
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000013
-
[hydroxymethylglutaryl-CoA reductase]-phosphate for the cytosolic isoenzyme Ia Rattus norvegicus
0.0000065
-
[hydroxymethylglutaryl-CoA reductase]-phosphate for the cytosolic isoenzyme IIa Rattus norvegicus
0.0000195
-
[hydroxymethylglutaryl-CoA reductase]-phosphate for the catalytic subunit of ca. 35000 Da Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
310000
-
cytosolic isoenzyme IIa, gel chromatography Rattus norvegicus
480000
-
cytosolic isoenzyme Ia, gel chromatography Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O Rattus norvegicus
-
?
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol after treatment with this solvent a catalytic subunit of 32000-35000 Da is obtained, activity increased Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
liver Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
310 315 no comments on grade of purification, native cytosolic isoenzyme Ia Rattus norvegicus
670 710 no comments on grade of purification, native cytosolic isoenzyme IIa Rattus norvegicus
21480
-
purification 4500fold, catalytic subunit of ~35000 Da after ethanol treatment Rattus norvegicus

Storage Stability

Storage Stability Organism
-40°C, several months, no measurable loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
Rattus norvegicus [hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
-
?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
Rattus norvegicus ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 for cytosolic isoenzymes Ia and IIa Rattus norvegicus
6 6.5 for the catalytic subunit of ~35000 Da retrieved after ethanol treatment Rattus norvegicus