BRENDA - Enzyme Database
show all sequences of 3.1.3.47

Inactivation of rat liver HMG-CoA reductase phosphatases by polycarboxylic acids

Hegardt G.; Gil G.; Calvet E.; J. Lipid Res. 24, 821-830 (1983)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-hydroxy-3-methylglutarate
-
Rattus norvegicus
citrate
-
Rattus norvegicus
diphosphate
-
Rattus norvegicus
Maleate
-
Rattus norvegicus
malonate
-
Rattus norvegicus
oxalate
-
Rattus norvegicus
phosphate
-
Rattus norvegicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
Rattus norvegicus
Mn2+
activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
310000
-
cytosolic isoenzyme IIa, gel chromatography
Rattus norvegicus
480000
-
cytosolic isoenzyme Ia, gel chromatography
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
Rattus norvegicus
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
liver
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
310
315
no comments on grade of purification, native cytosolic isoenzyme Ia
Rattus norvegicus
670
710
no comments on grade of purification, native cytosolic isoenzyme IIa
Rattus norvegicus
21480
-
purification 4500fold, catalytic subunit of ~35000 Da after ethanol treatment
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycogen synthase phosphate + H2O
-
94957
Rattus norvegicus
glycogen synthase + phosphate
-
94957
Rattus norvegicus
?
phosphorylase a phosphate + H2O
-
94957
Rattus norvegicus
phosphorylase a + phosphate
-
94957
Rattus norvegicus
?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94957
Rattus norvegicus
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
-
94957
Rattus norvegicus
?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94957
Rattus norvegicus
?
-
-
-
-
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-hydroxy-3-methylglutarate
-
Rattus norvegicus
citrate
-
Rattus norvegicus
diphosphate
-
Rattus norvegicus
Maleate
-
Rattus norvegicus
malonate
-
Rattus norvegicus
oxalate
-
Rattus norvegicus
phosphate
-
Rattus norvegicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
Rattus norvegicus
Mn2+
activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
310000
-
cytosolic isoenzyme IIa, gel chromatography
Rattus norvegicus
480000
-
cytosolic isoenzyme Ia, gel chromatography
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
Rattus norvegicus
-
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
liver
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
310
315
no comments on grade of purification, native cytosolic isoenzyme Ia
Rattus norvegicus
670
710
no comments on grade of purification, native cytosolic isoenzyme IIa
Rattus norvegicus
21480
-
purification 4500fold, catalytic subunit of ~35000 Da after ethanol treatment
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycogen synthase phosphate + H2O
-
94957
Rattus norvegicus
glycogen synthase + phosphate
-
94957
Rattus norvegicus
?
phosphorylase a phosphate + H2O
-
94957
Rattus norvegicus
phosphorylase a + phosphate
-
94957
Rattus norvegicus
?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94957
Rattus norvegicus
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
-
94957
Rattus norvegicus
?
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94957
Rattus norvegicus
?
-
-
-
-
Other publictions for EC 3.1.3.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
94956
Sitges M.; Gil G.; Hegardt G.
Partial purification from rat ...
Rattus norvegicus
J. Lipid Res.
25
497-506
1984
-
-
-
-
-
-
4
-
2
-
3
1
-
2
-
-
1
-
-
1
3
1
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
3
1
-
-
-
1
-
1
3
1
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
94957
Hegardt G.; Gil G.; Calvet E.
Inactivation of rat liver HMG- ...
Rattus norvegicus
J. Lipid Res.
24
821-830
1983
-
-
-
-
-
-
7
-
-
2
2
1
-
2
-
-
1
-
-
1
3
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
2
2
1
-
-
-
1
-
1
3
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94958
Brown W.E.; Rodwell V.W.
Purification and properties of ...
Rattus norvegicus
Biochim. Biophys. Acta
751
218-229
1983
-
-
-
-
-
-
2
-
1
-
2
1
2
1
-
-
1
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
2
1
2
-
-
1
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94959
Feingold K.R.; Wiley M.H.; Moser A.H.; Lear S.R.; Siperstein M.D.
Activation of HMG-CoA reductas ...
Rattus norvegicus
J. Lipid Res.
24
290-296
1983
-
-
-
-
-
-
2
-
4
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94960
Feingold K.R.; Wiley M.H.; Moser A.H.; Lear S.R.; Siperstein M.D.
Inhibition of HMG-CoA reductas ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
107
1376-1383
1982
-
-
-
-
-
-
2
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94961
Gil G.; Hegardt F.
Some properties of purified 3- ...
Rattus norvegicus
Arch. Biochem. Biophys.
214
192-198
1982
-
-
-
-
-
-
6
-
2
-
-
1
-
1
-
-
1
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
2
-
-
1
-
-
-
1
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94962
Gil G.; Sitges M.; Hegardt F.G.
Purification and properties of ...
Rattus norvegicus
Biochim. Biophys. Acta
663
211-221
1981
-
-
-
-
-
-
1
3
-
-
2
1
1
1
-
-
1
-
-
1
3
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
2
1
1
-
-
1
-
1
3
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
94963
Gil
Phosphorylation-dephosphorylat ...
Rattus norvegicus
FEBS Lett.
110
195-199
1980
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
1
-
-
1
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
1
-
1
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-