Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus | |
glycerol 1-phosphate | activation at high substrate concentrations | Rattus norvegicus | |
GTP | - |
Rattus norvegicus | |
phosphate | activates at high substrate concentrations | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,5-anhydro-D-mannitol 6-phosphate | - |
Rattus norvegicus | |
D-psicose 6-phosphate | poor inhibitor | Rattus norvegicus | |
D-ribose 5-phosphate | poor inhibitor | Rattus norvegicus | |
D-tagatose 6-phosphate | poor inhibitor | Rattus norvegicus | |
diethyldicarbonate | inactivation | Rattus norvegicus | |
fructose 6-phosphate | noncompetetive | Bos taurus | |
fructose 6-phosphate | noncompetetive | Rattus norvegicus | |
glycerol 1-phosphate | inhibition at low substrate concentrations | Rattus norvegicus | |
L-Sorbose 6-phosphate | - |
Rattus norvegicus | |
p-mercuribenzoate | - |
Rattus norvegicus | |
phosphate | - |
Bos taurus | |
phosphate | inhibition at low substrate concentrations | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
fructose 2,6-bisphosphate | - |
Rattus norvegicus | |
0.02 | - |
fructose 2,6-bisphosphate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
cytosol | - |
Bos taurus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration or sucrose density gradient centrifugation | Rattus norvegicus |
100000 | - |
gel filtration or sucrose density gradient centrifugation | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fructose 2,6-bisphosphate + H2O | Rattus norvegicus | - |
? | - |
? | |
fructose 2,6-bisphosphate + H2O | Bos taurus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
overview | - |
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase | Rattus norvegicus | |
beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | 40 | - |
Rattus norvegicus |
20 | 40 | - |
Bos taurus |
Storage Stability | Organism |
---|---|
-70°C for 3 months including 100 mM KCl, 0.5 mM dithiothreitol, 20% glycerol and 50 mM Tris-HCl at pH 7.4 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fructose 2,6-bisphosphate + H2O | - |
Rattus norvegicus | fructose 6-phosphate + phosphate | - |
? | |
fructose 2,6-bisphosphate + H2O | - |
Bos taurus | fructose 6-phosphate + phosphate | - |
? | |
fructose 2,6-bisphosphate + H2O | - |
Rattus norvegicus | ? | - |
? | |
fructose 2,6-bisphosphate + H2O | - |
Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000-55000, SDS-PAGE | Rattus norvegicus |
dimer | 2 * 50000-55000, SDS-PAGE | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
fructose 2,6-bisphosphate | - |
Rattus norvegicus |