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Literature summary for 3.1.3.46 extracted from

  • Cavalier, M.C.; Kim, S.G.; Neau, D.; Lee, Y.H.
    Molecular basis of the fructose-2,6-bisphosphatase reaction of PFKFB3: transition state and the C-terminal function (2012), Proteins, 80, 1143-1153.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.46

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with D-fructose 6-phosphate, diphosphate, or AlF4, sitting drop vapor diffusion method, using 100 mM Tris-HCl, pH 7.5, 20-25% (w/v) ethylene glycol, 200-400 mM tartaric acid, 5% (v/v) glycerol, and 12% (w/v) polyethylene glycol 4000 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-fructose 2,6-bisphosphate + H2O Homo sapiens
-
 D-fructose 6-phosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16875
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-fructose 2,6-bisphosphate + H2O
-
 Homo sapiens D-fructose 6-phosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
 Homo sapiens
PFKFB3 isoform Homo sapiens