Any feedback?
Literature summary for 3.1.3.45 extracted from
- Escherichia coli YrbI is 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (2003), J. Biol. Chem., 278, 18117-18123.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Escherichia coli |  |
| Cd2+ | - |
Escherichia coli | |
| Cu2+ | - |
Escherichia coli | |
| Hg2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.075 | - |
3-deoxy-D-manno-octulosonate 8-phosphate | pH 7.0, 37°C, presence of 1 mM Mg2+ | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 1 mM, little stimulation | Escherichia coli | |
| Co2+ | 1 mM, stimulation about 9fold | Escherichia coli | |
| Mg2+ | 1 mM, stimulation about 9fold | Escherichia coli | |
| Mn2+ | 1 mM, little stimulation | Escherichia coli | |
| additional information | divalent metal cofactor required | Escherichia coli | |
| Zn2+ | 1 mM, little stimulation | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19900 | - |
4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry | Escherichia coli |
| 23000 | - |
4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry | Escherichia coli |
| 89000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | last enzyme of 3-deoxy-D-manno-octulosonate biosynthetic pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-deoxy-D-manno-octulosonate 8-phosphate + H2O | high specificity | Escherichia coli | 3-deoxy-D-manno-octulosonate + phosphate | - |
? | |
| additional information | last enzyme of 3-deoxy-D-manno-octulosonate biosynthetic pathway | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 23000, SDS-PAGE, 4 * 19900, mass spectrometry | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | - |
Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | isoelectric focusing | 4.8 | 4.7 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
