Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bos taurus | |
Mg2+ | dependent | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, immunoblotting, truncated recombinant PDPc | Bos taurus |
50000 | - |
x * 50000, SDS-PAGE and immunoblotting, rPDPc | Bos taurus |
52625 | - |
x * 52625, calculated after PCR, subcloning and sequencing | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O | Bos taurus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O | - |
Bos taurus | [pyruvate dehydrogenase (lipoamide)] + phosphate | - |
ir | |
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O | - |
Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE and immunoblotting, rPDPc | Bos taurus |
? | x * 52625, calculated after PCR, subcloning and sequencing | Bos taurus |
? | x * 45000, immunoblotting, truncated recombinant PDPc | Bos taurus |