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Literature summary for 3.1.3.43 extracted from

  • Pettit, F.H.; Teague, W.M.; Reed, L.J.
    Pyruvate dehydrogenase phosphatase from bovine heart (1982), Methods Enzymol., 90, 402-407.
    View publication on PubMed
Search for more literature for 3.1.3.43

Inhibitors

Inhibitors Comment Organism Structure
F-
-
 Bos taurus
NADH
-
 Bos taurus
phosphate
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.058
-
 [Pyruvate dehydrogenase (lipoamide)]-phosphate
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Bos taurus
Mg2+ Km: 2 mM Bos taurus
Mn2+
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 97000 + 1 * 50000, SDS-PAGE, heterodimer Bos taurus
97000
-
 1 * 97000 + 1 * 50000, SDS-PAGE, heterodimer Bos taurus
150000
-
 sedimentation equilibrium and gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O Bos taurus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
assay method Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Bos taurus
-
kidney
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
 Bos taurus [pyruvate dehydrogenase (lipoamide)] + phosphate
-
 ir
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
 Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 97000 + 1 * 50000, SDS-PAGE, heterodimer Bos taurus