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Literature summary for 3.1.3.41 extracted from

  • Homareda, H.; Ushimaru, M.
    Stimulation of p-nitrophenylphosphatase activity of Na+/K+-ATPase by NaCl with oligomycin or ATP (2005), FEBS J., 272, 673-684.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl, cannot be substituted by nonhydrolyzable ATP analogues for stimulation Canis lupus familiaris
additional information stimulation mechanism and kinetics Canis lupus familiaris
oligomycin activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl Canis lupus familiaris

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Canis lupus familiaris
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl, gradually inhibits ATPase activity of the enzyme Canis lupus familiaris
Mg2+
-
Canis lupus familiaris
NaCl activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase, increases stimulation by other agents, e.g. KCl, oligomycin, and ATP, stimulation mechanism and kinetics, is also required for ATPase activity of the enzyme Canis lupus familiaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-nitrophenyl phosphate + H2O Canis lupus familiaris
-
4-nitrophenol + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially by microsome preperation Canis lupus familiaris

Source Tissue

Source Tissue Comment Organism Textmining
kidney medulla Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
4-nitrophenyl phosphatase activity of Na+/K+-ATPase in microsomes Canis lupus familiaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Canis lupus familiaris 4-nitrophenol + phosphate
-
?
additional information the canine Na+/K+-ATPase shows 4-nitrophenyl phosphatase activity Canis lupus familiaris ?
-
?

Synonyms

Synonyms Comment Organism
p-nitrophenylphosphatase
-
Canis lupus familiaris
PNPPase
-
Canis lupus familiaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
assay at Canis lupus familiaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 8.4 assay at pH 6.4, pH 7.4, and at pH 8.4 Canis lupus familiaris

pH Range

pH Minimum pH Maximum Comment Organism
6.4 8.4
-
Canis lupus familiaris