Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl, cannot be substituted by nonhydrolyzable ATP analogues for stimulation | Canis lupus familiaris | |
additional information | stimulation mechanism and kinetics | Canis lupus familiaris | |
oligomycin | activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl | Canis lupus familiaris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Canis lupus familiaris | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase only in presence of NaCl, gradually inhibits ATPase activity of the enzyme | Canis lupus familiaris | |
Mg2+ | - |
Canis lupus familiaris | |
NaCl | activates the 4-nitrophenyl phosphatase activity of the Na+/K+-ATPase, increases stimulation by other agents, e.g. KCl, oligomycin, and ATP, stimulation mechanism and kinetics, is also required for ATPase activity of the enzyme | Canis lupus familiaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | Canis lupus familiaris | - |
4-nitrophenol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially by microsome preperation | Canis lupus familiaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | medulla | Canis lupus familiaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
4-nitrophenyl phosphatase activity of Na+/K+-ATPase in microsomes | Canis lupus familiaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Canis lupus familiaris | 4-nitrophenol + phosphate | - |
? | |
additional information | the canine Na+/K+-ATPase shows 4-nitrophenyl phosphatase activity | Canis lupus familiaris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
p-nitrophenylphosphatase | - |
Canis lupus familiaris |
PNPPase | - |
Canis lupus familiaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Canis lupus familiaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 8.4 | assay at pH 6.4, pH 7.4, and at pH 8.4 | Canis lupus familiaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 8.4 | - |
Canis lupus familiaris |