Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Tween 20 | increases hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, little effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibition of hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, no effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid | Rattus norvegicus | |
Tween 20 | hydrolysis of phosphatidate bound to microsomal membrane in presence of optimal concentration of 8 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
phosphatidic acid | phosphatidate dispersed in sonicated microsomal lipid | Rattus norvegicus | |
0.16 | - |
phosphatidic acid | phosphatidate bound to microsomal membrane | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, optimal concentration: 8 mM, no effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidic acid + H2O | phosphatidate bound to microsomal membrane, phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes or phosphatidate dispersed in sonicated microsomal lipid | Rattus norvegicus | 1,2-diacyl-sn-glycerol + phosphate | - |
? |