Cloned (Comment) | Organism |
---|---|
functional overexpression as soluble His-tagged enzyme in Escherichia coli | Chlamydomonas sp. W80 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast stroma | - |
Arabidopsis thaliana | 9570 | - |
chloroplast stroma | - |
Chlamydomonas sp. W80 | 9570 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana | |
Mg2+ | required | Chlamydomonas sp. W80 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41600 | - |
x * 41600, recombinant enzyme, SDS-PAGE | Chlamydomonas sp. W80 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sedoheptulose 1,7-bisphosphate + H2O | Arabidopsis thaliana | the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle | sedoheptulose 7-phosphate + phosphate | - |
ir | |
sedoheptulose 1,7-bisphosphate + H2O | Chlamydomonas sp. W80 | the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle | sedoheptulose 7-phosphate + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Chlamydomonas sp. W80 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli in one step by nickel affinity chelating chromatography | Chlamydomonas sp. W80 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | expression profile of the enzyme in plant tissues, no activity and expression in roots | Arabidopsis thaliana | - |
silique | green | Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.224 | - |
leaf enzyme extract | Arabidopsis thaliana |
7.15 | - |
recombinant enzyme in the soluble fraction of Escherichia coli | Chlamydomonas sp. W80 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sedoheptulose 1,7-bisphosphate + H2O | - |
Arabidopsis thaliana | sedoheptulose 7-phosphate + phosphate | - |
ir | |
sedoheptulose 1,7-bisphosphate + H2O | - |
Chlamydomonas sp. W80 | sedoheptulose 7-phosphate + phosphate | - |
ir | |
sedoheptulose 1,7-bisphosphate + H2O | the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle | Arabidopsis thaliana | sedoheptulose 7-phosphate + phosphate | - |
ir | |
sedoheptulose 1,7-bisphosphate + H2O | the enzyme is involved in the generation of CO2-acceptor ribulose 1,5-bisphosphate in the Calvin cycle | Chlamydomonas sp. W80 | sedoheptulose 7-phosphate + phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 41600, recombinant enzyme, SDS-PAGE | Chlamydomonas sp. W80 |
Synonyms | Comment | Organism |
---|---|---|
SBPase | - |
Arabidopsis thaliana |
SBPase | - |
Chlamydomonas sp. W80 |
sedoheptulose-1,7-bisphosphatase | - |
Arabidopsis thaliana |
sedoheptulose-1,7-bisphosphatase | - |
Chlamydomonas sp. W80 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |
8 | - |
assay at | Chlamydomonas sp. W80 |