Literature summary for 3.1.3.36 extracted from

Cestari, I.; McLeland-Wieser, H.; Stuart, K.
Nuclear phosphatidylinositol 5-phosphatase is essential for allelic exclusion of variant surface glycoprotein genes in trypanosomes (2019), Mol. Cell. Biol., 39, e00395-18 .

Search for more literature for 3.1.3.36

Protein Variants

Protein Variants	Comment	Organism
D360A/N362A	significant decrease in catalytic activity	Trypanosoma brucei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0316	-	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate	mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication	Trypanosoma brucei
0.0738	-	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate	wild-type, pH not specified in the publication, temperature not specified in the publication	Trypanosoma brucei
0.088	-	1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate	wild-type, pH not specified in the publication, temperature not specified in the publication	Trypanosoma brucei

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Trypanosoma brucei	5634	-

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate + H2O	-	Trypanosoma brucei	1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + phosphate	-	?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O	-	Trypanosoma brucei	1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
tb927.11.6270	-	Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
28.2	-	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate	mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication	Trypanosoma brucei
57.5	-	1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate	wild-type, pH not specified in the publication, temperature not specified in the publication	Trypanosoma brucei
140.5	-	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate	wild-type, pH not specified in the publication, temperature not specified in the publication	Trypanosoma brucei

General Information

General Information	Comment	Organism
physiological function	nuclear phosphatidylinositol 5-phosphatase (PIP5Pase) interacts with repressor activator protein RAP1 in a multiprotein complex and functions in the control of variant surface glycoprotein allelic exclusion. RAP1 binds PIP5Pase substrate phosphatidylinositol 3,4,5-trisphosphate and catalytic mutation of PIP5Pase results in simultaneous transcription of variant surface glycoproteins from all telomeric expression sites and from silent subtelomeric variant surface glycoprotein arrays. PIP5Pase and RAP1 bind to telomeric expression sites, especially at 70-bp repeats and telomeres, and their binding is altered by PIP5Pase inactivation or knockdown	Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
653	-	1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate	wild-type, pH not specified in the publication, temperature not specified in the publication	Trypanosoma brucei
892	-	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate	mutant D360A/N362A, pH not specified in the publication, temperature not specified in the publication	Trypanosoma brucei
1900	-	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate	wild-type, pH not specified in the publication, temperature not specified in the publication	Trypanosoma brucei

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Release 2023.1 (January 2023)