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Literature summary for 3.1.3.36 extracted from

  • Choudhury, R.; Noakes, C.J.; McKenzie, E.; Kox, C.; Lowe, M.
    Differential clathrin binding and subcellular localization of OCRL1 splice isoforms (2009), J. Biol. Chem., 284, 9965-9973.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.36

Application

Application Comment Organism
molecular biology OCRL1 exists as two functional pools, one participating in clathrin-mediated trafficking events such as endocytosis, and another that is much less or not involved in this process Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae and in NRK- and HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
DELTA73-77 deletion of this LIDIA sequence motif within the N-terminal region of OCRL1 reveals that this sequence motif functions as a second clathrin binding domain in both isoforms Homo sapiens
DELTAPIP2 expression of OCRL1 isoform a, but not isoform b, lacking the 5-phosphatase domain impairs transferrin endocytosis Homo sapiens
G664D G664D mutation shows little effect on binding to clathrin, alpha-adaptin, Rac1 or APPL1, while binding to Rabs 5 and 6 is almost completely abolished. The effects of G664D mutation are the same for both OCRL1 isoforms Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Homo sapiens 5794
-
additional information OCRL1a is significantly more abundant in the cytoplasmic puncta, and there is less diffuse cytosolic staining compared to isoform b in NRK- and HeLa cells Homo sapiens
-
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perinuclear space both isoforms a and b predominantly localize to the perinuclear region Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens OCRL1 isoform a binds clathrin with higher affinity than isoform b, and is significantly more enriched in clathrin-coated trafficking intermediates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q01968
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information OCRL1 isoform a binds clathrin with higher affinity than isoform b, and is significantly more enriched in clathrin-coated trafficking intermediates Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
OCRL1a OCRL1 exists as two slice isoforms that differ by a single exon encoding 8 amino acids. The longer protein, termed isoform a, is the only form in brain, while both isoforms are present in all other tissues Homo sapiens