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Literature summary for 3.1.3.36 extracted from

  • Onnockx, S.; De Schutter, J.; Blockmans, M.; Xie, J.; Jacobs, C.; Vanderwinden, J.M.; Erneux, C.; Pirson, I.
    The association between the SH2-containing inositol polyphosphate 5-Phosphatase 2 (SHIP2) and the adaptor protein APS has an impact on biochemical properties of both partners (2008), J. Cell. Physiol., 214, 260-272.
    View publication on PubMed

Application

Application Comment Organism
molecular biology the interaction between adaptor protein APS and SHIP2 provides to both proteins potential negative regulatory mechanisms to act on the insulin cascade Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a HIS-tagged fusion protein in 3T3-L1 adipocytes and in CHO-IR cells (Chinese hamster ovary cells stably transfected with the insulin receptor) Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information it is shown hat SHIP2 interacts with the adaptor protein APS in 3T3-L1 adipocytes and in transfected CHO-IR cells (Chinese hamster ovary cells stably transfected with the insulin receptor). SHIP2 negatively regulates APS insulin-induced tyrosine phosphorylation and consequently inhibits APS association with c-Cbl. Co-transfection of SHIP2 and APS in CHO-IR cells further increases the inhibitory effect of SHIP2 on Akt insulin-induced phosphorylation Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-
membrane upon insulin stimulation, the adaptor protein APS and SHIP2 are recruited to cell membranes as seen by immunofluorescence studies Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-colums Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
the adaptor protein APS, which specifically interacts with SHIP2, increases the PtdIns(3,4,5)P3 5-phosphatase activity of SHIP2 (76.7% at 3 microM APS and by 111.3% at 6 microM APS) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-myo-phosphatidylinositol 3,4,5-trisphosphate
-
Homo sapiens D-myo-phosphatidylinositol 3,4-bisphosphate + phosphate
-
?

Synonyms

Synonyms Comment Organism
SH2-containing inositol polyphosphate 5-phosphatase 2
-
Homo sapiens
SHIP2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens