Application | Comment | Organism |
---|---|---|
molecular biology | the interaction between adaptor protein APS and SHIP2 provides to both proteins potential negative regulatory mechanisms to act on the insulin cascade | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed as a HIS-tagged fusion protein in 3T3-L1 adipocytes and in CHO-IR cells (Chinese hamster ovary cells stably transfected with the insulin receptor) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | it is shown hat SHIP2 interacts with the adaptor protein APS in 3T3-L1 adipocytes and in transfected CHO-IR cells (Chinese hamster ovary cells stably transfected with the insulin receptor). SHIP2 negatively regulates APS insulin-induced tyrosine phosphorylation and consequently inhibits APS association with c-Cbl. Co-transfection of SHIP2 and APS in CHO-IR cells further increases the inhibitory effect of SHIP2 on Akt insulin-induced phosphorylation | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
membrane | upon insulin stimulation, the adaptor protein APS and SHIP2 are recruited to cell membranes as seen by immunofluorescence studies | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-colums | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the adaptor protein APS, which specifically interacts with SHIP2, increases the PtdIns(3,4,5)P3 5-phosphatase activity of SHIP2 (76.7% at 3 microM APS and by 111.3% at 6 microM APS) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-myo-phosphatidylinositol 3,4,5-trisphosphate | - |
Homo sapiens | D-myo-phosphatidylinositol 3,4-bisphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SH2-containing inositol polyphosphate 5-phosphatase 2 | - |
Homo sapiens |
SHIP2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |