Literature summary for 3.1.3.36 extracted from

van Zeijl, L.; Ponsioen, B.; Giepmans, B.N.; Ariaens, A.; Postma, F.R.; Varnai, P.; Balla, T.; Divecha, N.; Jalink, K.; Moolenaar, W.H.
Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphate (2007), J. Cell Biol., 177, 881-891.

Search for more literature for 3.1.3.36

Application

Application	Comment	Organism
molecular biology	translocatable phosphatase: method is based on the rapamycin-induced heterodimerization of FRB (fragment of mammalian target of rapamycin that binds FKB12) and FKB12 (FK506 binding protein 12). A mutant version of 5-ptase with a defective membrane targeting domain (CAAX box) is fused to FKB12 and tagged with monomeric red fluorescent protein, whereas its binding partner FRB (fused to CFP) is tethered to the plasma membrane through palmitoylation. In the absence of rapamycin, 5-ptase resides in the cytosol and leaves D-myo-phosphatidylinositol 4,5-bisphosphate levels at the plasma membrane unaltered. Upon addition of 100 nM rapamycin, FKB12 and FRB undergo heterodimerization and the 5-ptase is recruited to the plasma membrane	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	using the translocatable 5-phosphatase method, it is shown that upon addition of rapamycin, which causes a rapid depletion of D-myo-phosphatidylinositol 4,5-bisphosphate, Cx43 gap junctional communication is closed	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-myo-phosphatidylinositol 4,5-bisphosphate	-	Homo sapiens	D-myo-phosphatidylinositol 4-phosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
5-ptase	-	Homo sapiens
PIP2 5-phosphatase	-	Homo sapiens
type IV phosphoinositide 5-phoshatase	-	Homo sapiens

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Release 2023.1 (January 2023)