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Literature summary for 3.1.3.36 extracted from
- A secreted salivary inositol polyphosphate 5-phosphatase from a blood-feeding insect: allosteric activation by soluble phosphoinositides and phosphatidylserine (2006), Biochemistry, 45, 5450-5457.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-dioctanoylphosphatidylinositol 4-phosphate | hydrolysis of D-myo-phosphatidylinositol 4,5-bisphosphate and D-myo-phosphatidylinositol 3,4,5-trisphosphate is stimulated up to 100fold | Rhodnius prolixus |  |
| dioctanoylphosphatidylserine | - |
Rhodnius prolixus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| IPPRp is cloned into the pET17b expression vector and moved into Escherichia coli strain BL21(DE3)pLys S for protein production | Rhodnius prolixus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | enzyme is inactive in the presence of 1 mM Ca2+ | Rhodnius prolixus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | with soluble diC8 D-myo-phosphatidylinositol 4,5-bisphosphate as a substrate, sigmoidal kinetics are observed, suggesting the presence of allosteric activation sites | Rhodnius prolixus | |
| 0.421 | - |
1D-myo-inositol 1,4,5-trisphosphate | - |
Rhodnius prolixus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Rhodnius prolixus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36500 | - |
deduced from cDNA | Rhodnius prolixus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodnius prolixus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification by gel filtration chromatography on Sephacryl S-100 followed by cation exchange chromatography on SP-Sepharose | Rhodnius prolixus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| saliva | - |
Rhodnius prolixus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1D-myo-inositol 1,4,5-trisphosphate + H2O | - |
Rhodnius prolixus | 1D-myo-inositol 1,4-bisphosphate + phosphate | - |
? | |
| D-myo-phosphatidylinositol 3,4,5-trisphosphate | - |
Rhodnius prolixus | D-myo-phosphatidylinositol 3,4-bisphosphate + phosphate | - |
? | |
| D-myo-phosphatidylinositol 4,5-bisphosphate | D-myo-phosphatidylinositol 4,5-bisphosphate is a much better substrate than D-myo-phosphatidylinositol 3,4,5-trisphosphate | Rhodnius prolixus | D-myo-phosphatidylinositol 4-phosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inositol polyphosphate 5-phosphatase | - |
Rhodnius prolixus |
| IPPRp | - |
Rhodnius prolixus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rhodnius prolixus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rhodnius prolixus |
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