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Literature summary for 3.1.3.34 extracted from

  • Becker, A.; Hurwitz, J.
    The enzymatic cleavage of phosphate termini from polynucleotides (1967), J. Biol. Chem., 242, 936-950.
    View publication on PubMed
Search for more literature for 3.1.3.34

Inhibitors

Inhibitors Comment Organism Structure
p-hydroxymercuribenzoate
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can replace Mg2+ Escherichia coli
Mg2+ required for maximal activity Escherichia coli
additional information effect of divalent cations alone or in combination Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside 3'-phosphate + H2O Escherichia coli
-
 deoxynucleoside + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 induced by bacteriophages /T4am82
-

Purification (Commentary)

Purification (Comment) Organism
infected with T4am82 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
380
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-15°C, 6 months, glycerol, less than 10% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside 3'-phosphate + H2O
-
 Escherichia coli deoxynucleoside + phosphate
-
 ?
pTp + H2O
-
 Escherichia coli pT + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6 6 sodium succinate buffer Escherichia coli
5.8 6.2 Tris-maleate buffer Escherichia coli
6 6.4 sodium cacodylate buffer Escherichia coli