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Literature summary for 3.1.3.3 extracted from
- Discovery and analysis of cofactor-dependent phosphoglycerate mutase homologs as novel phosphoserine phosphatases in Hydrogenobacter thermophilus (2012), J. Biol. Chem., 287, 11934-11941.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Hydrogenobacter thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibitory: EDTA at 10 mM; not inhibitory: EDTA at 10 mM | Hydrogenobacter thermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
L-phosphoserine | pH 8.0, 70°C | Hydrogenobacter thermophilus |  |
| 1.6 | - |
L-phosphoserine | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no divalent metal ion required | Hydrogenobacter thermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
1 * 24500, protein PspA, plus 1 * 24000, protein PspB, SDS-PAGE | Hydrogenobacter thermophilus |
| 24300 | - |
2 * 24300, SDS-PAGE | Hydrogenobacter thermophilus |
| 24500 | - |
1 * 24500, protein PspA, plus 1 * 24000, protein PspB, SDS-PAGE | Hydrogenobacter thermophilus |
| 37000 | - |
gel filtration | Hydrogenobacter thermophilus |
| 46000 | - |
gel filtration | Hydrogenobacter thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hydrogenobacter thermophilus | D3DFG8 | - |
- |
| Hydrogenobacter thermophilus | D3DFP8 | isoform pspB | - |
| Hydrogenobacter thermophilus DSM 6534 | D3DFG8 | - |
- |
| Hydrogenobacter thermophilus DSM 6534 | D3DFP8 | isoform pspB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hydrogenobacter thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28 | - |
substrate L-phosphoserine, pH 8.0, 70°C | Hydrogenobacter thermophilus |
| 58 | - |
substrate L-phosphoserine, pH 8.0, 70°C | Hydrogenobacter thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phosphoserine + H2O | - |
Hydrogenobacter thermophilus | L-serine + phosphate | strict substrate specificity for L-phosphoserine | ? | |
| L-phosphoserine + H2O | - |
Hydrogenobacter thermophilus DSM 6534 | L-serine + phosphate | strict substrate specificity for L-phosphoserine | ? | |
| additional information | poor substrates: D-phosphoserine, DL-phosphothreonine, 3-phosphoglycerate, 4-nitrophenylphosphate, and fructose-6-phosphate. Enzyme does not display phosphoglycerate mutase activity | Hydrogenobacter thermophilus | ? | - |
? | |
| additional information | poor substrates: D-phosphoserine, DL-phosphothreonine, 3-phosphoglycerate, 4-nitrophenylphosphate, and fructose-6-phosphate. Enzyme does not display phosphoglycerate mutase activity | Hydrogenobacter thermophilus DSM 6534 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 24500, protein PspA, plus 1 * 24000, protein PspB, SDS-PAGE | Hydrogenobacter thermophilus |
| dimer | 2 * 24300, SDS-PAGE | Hydrogenobacter thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pspA | - |
Hydrogenobacter thermophilus |
| pspB | - |
Hydrogenobacter thermophilus |
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