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Literature summary for 3.1.3.27 extracted from
- Phosphatidyl glycerophosphate phosphatase (1967), J. Lipid Res., 8, 456-462.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | distinctly stimulatory at 1 mM | Escherichia coli |  |
| SDS | - |
Escherichia coli | |
| Triton X-100 | optimal at 1-1.5 mg/ml | Escherichia coli | |
General Stability
| General Stability | Organism |
|---|---|
| heat labile | Escherichia coli |
| unstable at room temperature | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | higher concentrations | Escherichia coli | |
| Hg2+ | - |
Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
| NaF | - |
Escherichia coli | |
| Octadecylamine | - |
Escherichia coli | |
| phosphate | little effect up to 10 mM | Escherichia coli | |
| trimethyloctadecyl ammonium chloride | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
phosphatidylglycerophosphate | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylglycerophosphate + H2O | Escherichia coli | biosynthesis of phospholipids | ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Triton X-100 | - |
Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.028 | - |
partially purified | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for weeks | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylglycerophosphate + H2O | hydrolysis of other simple phosphomonoesters not catalyzed | Escherichia coli | phosphatidylglycerol + phosphate | - |
? | |
| phosphatidylglycerophosphate + H2O | biosynthesis of phospholipids | Escherichia coli | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | little activity below pH 7.0 and above pH 8.0 | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
95% activity for several weeks, stored in liquid nitrogen | Escherichia coli |
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Release 2023.1 (January 2023)
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