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Literature summary for 3.1.3.27 extracted from
- Crystal structure of lipid phosphatase Escherichia coli phosphatidylglycerophosphate phosphatase B (2014), Proc. Natl. Acad. Sci. USA, 111, 7636-7640.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| vapor diffusion method | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A83F | the mutant shows reduced activity | Escherichia coli |
| D211E | the mutant shows strongly reduced activity | Escherichia coli |
| F61W | the mutant shows reduced activity | Escherichia coli |
| G89L | the mutant shows strongly reduced activity | Escherichia coli |
| G91M | the mutant shows reduced activity | Escherichia coli |
| H163A | the mutant shows strongly reduced activity | Escherichia coli |
| H207A | the mutant shows strongly reduced activity | Escherichia coli |
| H57F | the mutant shows about wild type activity | Escherichia coli |
| K93E | the mutant shows reduced activity | Escherichia coli |
| K97A | the mutant shows strongly reduced activity | Escherichia coli |
| L58F | the mutant shows about wild type activity | Escherichia coli |
| Q90A | the mutant shows reduced activity | Escherichia coli |
| R104A | the mutant shows strongly reduced activity | Escherichia coli |
| R201A | the mutant shows strongly reduced activity | Escherichia coli |
| V54F | the mutant shows reduced activity | Escherichia coli |
| V88F | the mutant shows reduced activity | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is stabilized by Na2MoO4 but not by Na2SO4 | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
1,2-dioleoyl-sn-glycero-3-phosphate | pH and temperature not specified in the publication | Escherichia coli |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | trans-membrane protein | Escherichia coli | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| undecaprenyl diphosphate + H2O | Escherichia coli | - |
? | - |
? | |
| undecaprenyl diphosphate + H2O | Escherichia coli B / ATCC 11303 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | A0A140N9T3 | - |
- |
| Escherichia coli B / ATCC 11303 | A0A140N9T3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified in a detergent combination of nonyl-beta-D-glucopyranoside and n-dodecyl-N,N-dimethylamine-N-oxide | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dioctanoyl-sn-glycero-3-diphosphate + H2O | - |
Escherichia coli | ? | - |
? | |
| 1,2-dioctanoyl-sn-glycero-3-diphosphate + H2O | - |
Escherichia coli B / ATCC 11303 | ? | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-diphosphate + H2O | - |
Escherichia coli | ? | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-diphosphate + H2O | - |
Escherichia coli B / ATCC 11303 | ? | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-phosphate + H2O | highest activity | Escherichia coli | 1,2-dioleoyl-sn-glycerol + phosphate | - |
? | |
| 1,2-dioleoyl-sn-glycero-3-phosphate + H2O | highest activity | Escherichia coli B / ATCC 11303 | 1,2-dioleoyl-sn-glycerol + phosphate | - |
? | |
| 1-oleoyl-2-lyso-sn-glycero-3-phosphate + H2O | - |
Escherichia coli | 1-oleoyl-2-lyso-sn-glycerol + phosphate | - |
? | |
| 1-oleoyl-2-lyso-sn-glycero-3-phosphate + H2O | - |
Escherichia coli B / ATCC 11303 | 1-oleoyl-2-lyso-sn-glycerol + phosphate | - |
? | |
| undecaprenyl diphosphate + H2O | - |
Escherichia coli | ? | - |
? | |
| undecaprenyl diphosphate + H2O | - |
Escherichia coli B / ATCC 11303 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PgpB | - |
Escherichia coli |
| phosphatidylglycerophosphate phosphatase B | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43 | - |
the melting temperature of the wild type enzyme is 43°C | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 24 | - |
1,2-dioleoyl-sn-glycero-3-phosphate | pH and temperature not specified in the publication | Escherichia coli | |
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