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Literature summary for 3.1.3.26 extracted from
- A calcium-activated phytase from pollen of Lilium longiflorum (1986), Plant Physiol., 82, 333-335.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | at concentrations higher than substrate concentration, pH 8 phytase | Lilium longiflorum |  |
| Cu2+ | pH 5 phytase | Lilium longiflorum | |
| EDTA | - |
Lilium longiflorum | |
| Mg2+ | pH 8 phytase | Lilium longiflorum | |
| Mn2+ | pH 8 phytase | Lilium longiflorum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0072 | - |
myo-inositol hexakisphosphate | - |
Lilium longiflorum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2 mM, pH 8 phytase, more than 300% activation at concentrations equimolar to the substrate, pH 8 | Lilium longiflorum | |
| Sr2+ | activates | Lilium longiflorum | |
| Sr2+ | 2 mM, pH 8 phytase, about 250% activation at pH 8 | Lilium longiflorum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
pH 8 phytase, gel filtration | Lilium longiflorum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lilium longiflorum | - |
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
| Lilium longiflorum | - |
Thunb. Cv. Nellie White, 2 enzyme forms pH 5 phytase and pH 8 phytase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Lilium longiflorum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pollen | - |
Lilium longiflorum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.066 | - |
- |
Lilium longiflorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | - |
Lilium longiflorum | myo-inositol pentakisphosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 60 | - |
Lilium longiflorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Lilium longiflorum |
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