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Literature summary for 3.1.3.26 extracted from

  • Sutardy; Buckle, K.A.
    The characteristics of soybean phytase (1986), J. Food Biochem., 10, 197-216.
No PubMed abstract available
Search for more literature for 3.1.3.26

General Stability

General Stability Organism
the enzyme can withstand repeated freezing and thawing without appreciable loss of acticity Glycine max

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Glycine max
Al3+ 1 mM, slight inhibition Glycine max
Ca2+ 1 mM, slight inhibition Glycine max
citrate
-
 Glycine max
Co2+ 1 mM, slight inhibition Glycine max
Cu2+ 1 mM, 23% inhibition Glycine max
EDTA
-
 Glycine max
F- NaF Glycine max
glutathione
-
 Glycine max
Hg2+ 1 mM, 22% inhibition Glycine max
iodoacetamide
-
 Glycine max
L-ascorbic acid
-
 Glycine max
L-Cys
-
 Glycine max
Mg2+ 1 mM, slight inhibition Glycine max
Mn2+ 1 mM, slight inhibition Glycine max
myo-inositol hexakisphosphate at high concentrations Glycine max
NEM
-
 Glycine max
Ni2+ 1 mM, slight inhibition Glycine max
oxalate
-
 Glycine max
Tartrate
-
 Glycine max
Zn2+ 1 mM, 25% inhibition Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 myo-inositol hexakisphosphate
-
 Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

Purification (Comment) Organism
partial Glycine max

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.53
-
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Glycine max myo-inositol pentakisphosphate + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Glycine max

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 about 50% of maximal activity at 40°C and at 70°C Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, stable up to Glycine max
60
-
 10 min, 5% loss of activity Glycine max
70
-
 10 min, about 50% loss of activity Glycine max
90
-
 10 min, complete inactivation Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
4.2 5.3 pH 4.2: about 70% of maximal activity, pH 5.3: about 50% of maximal activity Glycine max